物理化学学报 >> 2000, Vol. 16 >> Issue (08): 764-768.doi: 10.3866/PKU.WHXB20000818
研究简报 上一篇
郇宇, 褚德萤, 汤勇, 曹炜
收稿日期:
修回日期:
发布日期:
通讯作者:
Huan Yu, Chu De-Ying, Tang Yong, Cao Wei
Received:
Revised:
Published:
Contact:
关键词: 金属硫蛋白, 镉离子, 铅离子, 等温微量滴定量热
Abstract:
Metallothionein, a class of low molecular weight, metal- and cysteine-rich Proteins, are postulated to play a central role in metal-related biological processes such as the detoxification of Cd2+and Pb2+. The thermal Properties of the interactions of rabbit liver Zn_7MT2 and apo-MT2 with Cd2+ and Pb2+ were studied using isothermal titration calorimeter. The same product Cd_7MT formed in the reactions of Cd2+ with Zn_7MT2 and apo-MT2, while the reactions of Ph2+ with Zn_7MT2 and apo-MT2 Produced two MT species Ph_7MT and Ph_7MT'. The thermodynamic parameters (△H、 △G、 △S, etc. ) for the reactions of MT with 1 mol metal ions were measured at 293.15 K, pH 4.70. All of these reactions are spontaneous, exothermic processes. The values of △G and K show that Cd2+ is more affinitive to MT than Ph2+. The equilibrium constants K of the replacement reactions of Zn_7MT2 by 1 mol Cd2+ and Pb2+ are 2. 7×105 and 1. 3×105 respectively, that means the concentrations of free Cd2+ and Pb2+ are depressed to about 1. 0×10-5 after the reactions with Zn_7MT2. These data can be used to evaluate quantitatively the ability of MT in Cd2+ and Pb2+ detoxificatio.
Key words: Metallothionein, Cadmium(II) ion, Lead(II) ion, Isothermal titration calorimetry
郇宇, 褚德萤, 汤勇, 曹炜. 金属硫蛋白与Cd2+、Pb2+络合反应的微量量热滴定[J]. 物理化学学报, 2000, 16(08): 764-768.
Huan Yu, Chu De-Ying, Tang Yong, Cao Wei. Microcalorimetry Study on the Reaction of Metallothionein with Cd2+ and Pb2+[J]. Acta Phys. -Chim. Sin., 2000, 16(08): 764-768.
0 /
导出引用管理器 EndNote (中文内容)|EndNote (英文内容)| Reference Manager|ProCite|BibTeX|RefWorks
链接本文: http://www.whxb.pku.edu.cn/CN/10.3866/PKU.WHXB20000818
http://www.whxb.pku.edu.cn/CN/Y2000/V16/I08/764
Cited