物理化学学报 >> 2002, Vol. 18 >> Issue (08): 741 -745 .doi: 10.3866/PKU.WHXB20020814

研究简报 上一篇    下一篇

钙调素修饰金电极的交流阻抗行为研究

丁克强;贺晓凌;王庆飞;蒋殿录;白娟;童汝亭;王心葵   

  1. 河北师范大学化学学院,石家庄 050016; 中国科学院山西煤炭化学研究所,太原 030001
  • 收稿日期:2001-12-17 修回日期:2002-02-10 发布日期:2002-08-15
  • 通讯作者: 丁克强 E-mail:dkeqiang@263.net

The AC Impedance Study of Calmodulin Modified on Au Electrode

Ding Ke-Qiang;He Xiao-Ling;Wang Qing-Fei;Jiang Dian-Lu;Bai Juan;Tong Ru-Ting;Wang Xin-Kui   

  1. Chemistry College, Hebei Normal University, Shijiazhuang 050016;Institute of Coal Chemistry, Chinese Academy of Science, Taiyuan 030001
  • Received:2001-12-17 Revised:2002-02-10 Published:2002-08-15
  • Contact: Ding Ke-Qiang E-mail:dkeqiang@263.net

摘要: 利用交流阻抗方法, 以氧化还原对为探针,对自组装到金电极表面的钙调素 (CaM)膜的成膜过程、不同pH值的影响以及与钙离子的结合情况进行了较系统的研究.实验结果表明, 钙调素可以自组装到金电极表面并且其自组装过程较为明显地分为快、慢两个步骤;还发现钙调素膜的性能结构随体系不同的pH值发生有规律的变化,在其等电点,膜的结构最致密;而且钙调素膜与钙离子的结合也分为较明显的三个阶段.

关键词: 钙调素(CaM), 自组装, pH值, 钙离子, 交流阻抗

Abstract: Calmodulin(CaM) as a major intracellular Ca2+ receptor, was self-assembled on Au electrode and investigated by electrochemical impedance spectroscopy(EIS). The results demonstrated that the self-assembly process of CaM could be divided into rapid and slow steps clearly. The influence of pH value on the new modified electrode was found to be regular, and the most packed structure appeared at the calmodulin’s isoelectric-point, the combining process of calmodulin with Ca2+ was found to be composed of three stages. In conclusion, our AC impedance study of CaM is meaningful to reveal the mechanism of CaM in the real life system.

Key words: Calmodulin, Self-assembly, pH value, Ca2+, AC impedance