物理化学学报 >> 1994, Vol. 10 >> Issue (07): 648-653.doi: 10.3866/PKU.WHXB19940714

研究简报 上一篇    下一篇

邻菲啰啉对固定尿酸酶催化反应动力学的影响

阚锦晴, 穆绍林   

  1. 扬州大学师范学院化学系,扬州 225002
  • 收稿日期:1993-02-25 修回日期:1993-06-28 发布日期:1994-07-15
  • 通讯作者: 阚锦晴

The Effect of O-phenanthroline on the Kinetics of the Immobilized Uricase Catalyzed Reaction

Kan Jin-Qing , Mu Shao-Lin   

  1. Department of Chemistry, Teachers College, Yangzhou University, Yangzhou 225002
  • Received:1993-02-25 Revised:1993-06-28 Published:1994-07-15
  • Contact: Kan Jin-Qing

关键词: 尿酸酶电极, 激活剂, 酶催化反应动力学

Abstract:

The activation energy of the enzyme-catalyzed reaction for uric acid decreases markedly in the presence of o-phenanthroline, which activates the bioelectrochemicla activity of the polypyrrole uricase electrode. The response current of the enzyme electrodeis independent of the concentration of o-phenanthroline. Based on the experimental results, the mechamsm of the enzyme-catalyzed reaction for uric acid in the presence of o-phenanthroline is presented as follows: E+A→EA, EA+S EAS, EAS→EA+P, where E, A, S and P are the enzyme, activator, substrate and product, respectively. The effects of pH value, potential and the uric acid concentration on the response currents of the uricase electrode have been studied in the presence of o-phenanthroline. In the presence of o-phenanthroline, the response current of the enzyme electrode increase linearly with increasing concentration of uric acid in the region of 0.07 to 0.67 mmol·L~(-1), therefore the polypyrrole uricase electrode which has once lost its activity can be activated and used again to determine the substrate concentration.

Key words: Uricase electrode, Activator, Kinetics of the enzyme-catalyzed reaction