物理化学学报 >> 2006, Vol. 22 >> Issue (02): 254-260.doi: 10.3866/PKU.WHXB20060225

综述 上一篇    

光谱法研究蛋白质与表面活性剂的相互作用

吴丹; 徐桂英   

  1. 山东大学胶体与界面化学教育部重点实验室, 济南 250100
  • 收稿日期:2005-07-08 修回日期:2005-08-22 发布日期:2006-01-22
  • 通讯作者: 徐桂英 E-mail:xuguiying@sdu.edu.cn

Study on Protein-Surfactant Interaction by Spectroscopic Methods

WU Dan; XU Gui-ying   

  1. Key Laboratory of Colloid & Interface Chemistry, Ministry of Education, Shandong University, Jinan 250100, P. R. China
  • Received:2005-07-08 Revised:2005-08-22 Published:2006-01-22
  • Contact: XU Gui-ying E-mail:xuguiying@sdu.edu.cn

摘要: 结合本课题组的工作, 较系统地总结了近年来有关紫外吸收光谱、荧光光谱、圆二色光谱和电子自旋共振光谱技术在蛋白质-表面活性剂混合体系研究中的应用. 大量研究表明, 借助于光谱技术不仅可以研究蛋白质结构与功能的关系, 而且可以探讨蛋白质与表面活性剂的作用机理.

关键词: 蛋白质, 表面活性剂, 紫外吸收光谱, 荧光光谱, 圆二色光谱, 电子自旋共振光谱, 相互作用

Abstract: Our results and the applications of spectroscopic methods in studying protein-surfactant system are systematically summarized. Those methods include UV absorption spectroscopy, fluorescence spectroscopy, circular dichroism and electron spin resonance. Many studies indicate that spectroscopic methods can be used to not only study the relationship between structure and function of protein but also probe the interaction mechanism of protein and surfactant.

Key words: Protein, Surfactant, UV absorption spectroscopy, Fluorescence spectroscopy, Circular dichroism, Electron spin resonance, Interaction