物理化学学报 >> 1996, Vol. 12 >> Issue (05): 476-479.doi: 10.3866/PKU.WHXB19960519

研究简报 上一篇    

水稻线粒体DNA的热变性及热力学研究

周培疆,谢昌礼,屈松生,周涵韬,朱英国   

  1. 武汉大学化学系,武汉 430072|武汉大学生命科学学院,武汉 430072
  • 收稿日期:1995-09-20 修回日期:1996-02-05 发布日期:1996-05-15
  • 通讯作者: 屈松生

Studies on Heat Denaturation and Thermodynamics of Rice Mitochondrial DNA

Zhou Pei-Jiang,Xie Chang-Li,Qu Song-Sheng,Zhou Han-Tao,Zhu Ying-Guo   

  1. Department of Chemistry,Wuhan University,Wuhan 430072|School of Life Science,Wuhan University,Wuhan 430072
  • Received:1995-09-20 Revised:1996-02-05 Published:1996-05-15
  • Contact: Qu Song-Sheng

关键词: 水稻线粒体DNA, 热变性, 紫外光谱, 热力学

Abstract:

The heat denaturation of the rice mitochondrial DNA in two different media has been studied by using ultraviolet absorption spectrometry over the temperature range 25-85 ℃ The heat denaturation curve of the rice mitochondrial DNA in 0.15 mol﹒L-1 NaCl solution has been obtained and the thermodynamic functions of the rice mitochondrial DNA in the heat denaturation process have been calculated. Furthermore, the relationship between these thermodynamic functions and the structure of the rice mitochondrial DNA has been discussed preliminarily.

Key words: Rice mitochondrial DNA, Heat denaturation, Ultraviolet spectrum, Thermodynamics