物理化学学报 >> 2020, Vol. 36 >> Issue (6): 1905051.doi: 10.3866/PKU.WHXB201905051

所属专题: 热分析动力学和热动力学

综述 上一篇    下一篇

生物热化学和热动力学研究进展

谢文1,周莲娇2,徐娟2,郭清莲1,蒋风雷2,刘义2,*()   

  1. 1 武汉大学中南医院检验科,武汉 430071
    2 武汉大学化学与分子科学学院,武汉 430072
  • 收稿日期:2019-05-14 录用日期:2019-06-19 发布日期:2019-12-18
  • 通讯作者: 刘义 E-mail:yiliuchem@whu.edu.cn
  • 作者简介:刘义,武汉大学化学与分子科学学院教授、博士生导师。2002年获教育部高校青年教师奖、国务院政府特殊津贴;2012年获国家杰出青年科学基金;2014年获批湖北省创新研究群体、首批武汉黄鹤英才(科技)计划。主要研究方向为生物热化学与热分析、纳米材料生物效应和靶向药物化学
  • 基金资助:
    国家自然科学基金(21873075);国家自然科学基金(21573168)

Advances in Biothermochemistry and Thermokinetics

Wen Xie1,Lianjiao Zhou2,Juan Xu2,Qinglian Guo1,Fenglei Jiang2,Yi Liu2,*()   

  1. 1 Department of Clinical Laboratory, Zhongnan Hospital, Wuhan University, Wuhan 430071, P. R. China
    2 College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China
  • Received:2019-05-14 Accepted:2019-06-19 Published:2019-12-18
  • Contact: Yi Liu E-mail:yiliuchem@whu.edu.cn
  • Supported by:
    the National Science Foundation of China(21873075);the National Science Foundation of China(21573168)

摘要:

生命相关过程伴随着极其复杂的化学和物理过程,包含着物质变化和能量转换,其中部分能量不可避免地会以热的形式表现出来。用微量热技术和热动力学方法,研究复杂生命体系和相关反应的热动力学过程,可宏观地、本质地反映生命相关过程的内在规律。本文综述了生物量热学方法和技术在生命科学中的应用,介绍了生物量热技术在生态系统、生物组织和器官、细胞水平、亚细胞水平和分子层面等不同生物层次和结构水平上的研究现状和进展。

关键词: 生物热化学, 热动力学, 微量热, 新陈代谢, 相互作用

Abstract:

Biological systems can be regarded as complex and open thermodynamic systems. All processes involved in biological growth and metabolism are accompanied by material and energy exchange. During metabolism, energy in the organisms is released in the form of heat, i.e., metabolic heat, which is the basis for development in the field of biothermochemistry. The calorimetric method considers the thermal effects produced by the various forms of action as the research object, to reveal the law of energy change and quantitative energy conversion. Studying the thermodynamic processes of complex biological systems and related reactions through microcalorimetry and thermodynamic methods reflects the intrinsic laws of life-related processes macroscopically and intrinsically. With the tremendous development and progress in microcalorimetry in terms of the temperature measurement accuracy, stability of temperature control, automation, and multi-functionalization, calorimetry has been widely used in life sciences. It can be used to describe macroscopic processes such as ecosystems and biological evolution, observe organismal and cell growth, examine mitochondrial metabolism, and study problems at the molecular level, including enzymatic reactions and interactions between small molecules and biomacromolecules. Herein, the application of biomass calorimetry in the life sciences is reviewed. The status and progress of biomass calorimetry at different biological and structural levels, such as the ecosystem, biological, organ, cellular, subcellular, and molecular levels are introduced. For example, soil microbial metabolic activity is a universal index for evaluating soil quality. The growth and metabolism of organisms as well as the physical and chemical processes of substances in soil are often accompanied by heat release, which is usually a nonselective signal. The use of isothermal microcalorimetry to nonspecifically monitor and record soil microbial metabolic characteristics has promoted the study of microbial metabolism in complex soil systems. The application of calorimetry to the study of tissues and organs mainly involves the calorimetric study of isolated animal and plant tissues and organs. Calorimetry of animal and microbial cells is considered the most common application of calorimetry in life sciences research. It mainly involves the classification and identification of bacteria, their growth and metabolism, inhibition mechanisms of drugs on microbial growth, principles of kinetics, and the thermodynamic characteristics of microbial growth and metabolism. However, owing to the lack of specificity of biomass calorimetry and the lack of direct access to information at the molecular level, more applications of calorimetry combined with other analytical techniques (especially in biology, medicine, and pharmacy) are needed in the future.

Key words: Biothermochemistry, Thermokinetics, Microcalorimetry, Metabolism, Interaction

MSC2000: 

  • O642