Acta Phys. -Chim. Sin. ›› 1994, Vol. 10 ›› Issue (01): 82-86.doi: 10.3866/PKU.WHXB19940119

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A Study of Intercalation Binding of Harmaline and Harmine to DNA by Microcalorimetry

Wang Bao-Huai, Zhang You-Min, Yang Ming, Miao Pei, Wang Kui   

  1. Institute of Physical Chemistry, Peking University, Beijing 100871| Research Laboratory of Natural and Biomimetic Drugs, Beijing Medical University, Beijing 100083
  • Received:1992-05-16 Revised:1992-10-22 Published:1994-01-15
  • Contact: Wang Bao-Huai

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Abstract:

The enthalpy changes for the binding of two alkaloids with the bioactivity to inhibit monoamine oxidase, harmaline(HL) and harmine(HM) to calf thymus DNA have been determined calorimetrically in a 0.01 mol·L~(-1) piperazine-N、N -bis(2-ethanesulfonic acid) buffer(pH=7.0) at 298.15 K by a MS-80 standard Calvet microcalorimeter. The results are △H_(298)(HL)=-10.7 kJ·mol~(-1), △H_(298)(HM)=-29.6 kJ·mol~(-1) respectively. According to the binding energy and thermokinetic curves from the experiments, the mechanism of intercalationm binding of two alkaloids to DNA has been discussed.

Key words: Alkaloid, Anticancer, Intercalation, Enthalpy change, Thermogram