Acta Phys. -Chim. Sin. ›› 1996, Vol. 12 ›› Issue (04): 353-356.doi: 10.3866/PKU.WHXB19960413

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Conformation and Activity of RNaseA in Dodecylammonium Butyrate-Cyclohexane Reverse Micellar Solution

Huang Wen,Li Xiao-Feng,Gu Ti-Ren   

  1. Laboratory of Chemistry,Beijing University of Aeronautics and Austronautucs,Beijing 100083
  • Received:1995-06-16 Revised:1995-09-03 Published:1996-04-15
  • Contact: Huang Wen

Abstract:

The activity and conformation of ribonuclerse A (RNaseA) solubilized in cyclohexane via dodecylammonium butyrate(DAB) reverse micelles were investigated. The activity of RNaseA was studied using the cytidine 2’,3’ -phosphate as the substrate, and it was found that kcat increases significantly with respect to that in water attended by an increased Km•FT-IR spectra of RNaseA in reverse micellar solution were investigated as a function of w0(= [H2O]/ [DAB]), and it was noted that the structure of RNaseA became losser in reverse micelles campared to that in aqueous solution. The relation between activity and conformation was discussed.

Key words: Bovine pancreatic ribonuclease A(RNaseA), Dodecylammonium butyrate, Reverse micelle, FT-IR spectra, Surfactant