Acta Phys. -Chim. Sin. ›› 1996, Vol. 12 ›› Issue (04): 377-381.doi: 10.3866/PKU.WHXB19960419

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Cell Kinetic Study II.Thermokinetic Study of the Process of Cell Growth Inhibited by Metabolites

Liu Yi, Tan An-Min, Xie Chang-Li, Wang Cun-Xin, Qu Song-Sheng, Hao Zong-Yu   

  1. Department of Chemistry,Wuhan University,Wuhan 430072|Henan Health Epidemic Prevention Station,Zhengzhou 450003
  • Received:1995-08-09 Revised:1995-11-18 Published:1996-04-15
  • Contact: Qu Song-Sheng

Abstract:

By using an LKB2277 Bioactivity Monitor, we have determined the thermogenesis power curves of four kinds of Brucellas S2(55007), M5 (55009),83-202,83-980. And established the thermokinetic equation for the process of cell growth inhibited by the products of metabolism, as:
Ln[1/(β﹒Pt)-1]=ln[1/(β﹒P0)-1]-k0﹒t
From this equation, the rate constants of cell growth k [= k0(1 - β﹒P0)] were obtained. This thermokinetic equation is very suitable for cell growth of separated culture and is very important for the study of bacterial limited growth and their characteristics.

Key words: Bacterial growth, Microcalorimetry, Thermokinetics, Thermochemistry