Acta Phys. -Chim. Sin. ›› 1997, Vol. 13 ›› Issue (07): 643-646.doi: 10.3866/PKU.WHXB19970714

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Studies on Model Compound of Purple Acid Phosphatases Hydrolysis of ATP

Wang Tian-Zhi,Wu Ding-Quan,Huang Zai-Yin,Qu Song-Sheng,Li Dong-Feng,Liao Zhan-Ru,Wan Hong-Wen   

  1. Department of Chemistry,Wuhan University,Wuhan 430072|Department of Chemistry,Central China Normal University,Wuhan 430070
  • Received:1996-11-12 Revised:1997-02-04 Published:1997-07-15
  • Contact: Qu Song-Sheng

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Abstract:

Studies on the thermokinetic properties of model compound of Purple acid phosphatases (Na2[Fe2(μ-O)(μ-OAC)2(IDA)2].5H2O) hydrolysis of ATP, at 37℃, pH=6.0 by using LKB-2107 Batch microcalorimentry system. The Michaelis constant Km=(2.898 ± 0.195)×10-4mol•L-1 and rate constant k2=(4.617±0.193)×10-3•s-1 have been determined. The result has been discussed.

Key words: Thermokinetic, Microcalorimetry, ATP, Hydrolysis