Acta Phys. -Chim. Sin. ›› 1997, Vol. 13 ›› Issue (11): 969-973.doi: 10.3866/PKU.WHXB19971103

• Communication • Previous Articles     Next Articles

Microcalorimetric Studies of the Tubulin Polymerization and the Interaxtion between Tubuin and Taxol

Han Gong-She,Yan Long-Fei,Wang Bao-Huai,Zhang You-Min   

  1. College of Biological Science,China Agricultural University,Beijing 100094|Department of Chemistry,Peking University,Beijing 100871
  • Received:1997-08-25 Revised:1997-09-22 Published:1997-11-15
  • Contact: Wang Bao-Huai

Abstract:

The heat properties of porcine brain tubulin polymerization and the interaction of the tubulin with taxol were studied with an MS-80 Calvet Microcalorimeter .The heat vibration occured within 1.5h when tubulin solution was diluted from a highe r concentration to a lower one. The thermodynamic parameters of tubulin polymerization at 303.15K and 298. 15K were measured in 10μmol•L-1 tubulin solution, its △H's were 22kJ•mol-1 and 29.3kJ•mol-1,respectively.Adding different concentration of taxol in the course of polymerization of tubulin give rise to a great change in △H. The time of taxol combining to polymerized microtubule was about one minute after the beginning of the reaction. The mechanism of tubulin polymerization under different conditions was discussed.

Key words: Tubulin, Taxol, Thermogram