Acta Phys. -Chim. Sin. ›› 2002, Vol. 18 ›› Issue (06): 490-494.doi: 10.3866/PKU.WHXB20020603

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Change of Adsorption State of Oligodeoxynucleotides on the Silver Electrode Surface with Change of Potential

Shen He-Bai;Zhou Wen-Jun;Yang Hai-Feng;Yu Pei-Tao   

  1. Chemistry Department of Life and Environmental Science College, Shanghai Normal University, Shanghai 200234
  • Received:2001-10-16 Revised:2002-01-08 Published:2002-06-15
  • Contact: Shen He-Bai

Abstract: The oligodeoxynucleotides is anchoraged on the silver electrode surface by means of physical methods. The in situ Electrochemistry-Surface Enhanced Raman Scattering (SERS) Spectrum is used to study the adsorption state of 26-mers oligodeoxynucleotides (ODN) and 13-mers ODN on the electrode surface respectively. The SERS spectra of oligdeoxynucleotides based on the silver electrode have obtained. The results of study of the SERS spectra change of ODN with the change of potential on the silver electrode demonstrate that the oligodeoxynucleotides adsorbed on the silver electrode surface primarily through the special base-Adenine (A).The adsorption state changes with the variation of the potential on the electrode. The shorter ODN changes more quickly and is more sensitive to the change of potential than the longer ODN(see Table 1).

Key words: Oligodeoxynucleotide (ODN), SERS, Adenine, Adsorption, Silver electrodes