Acta Phys. -Chim. Sin. ›› 2002, Vol. 18 ›› Issue (08): 741-745.doi: 10.3866/PKU.WHXB20020814

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The AC Impedance Study of Calmodulin Modified on Au Electrode

Ding Ke-Qiang;He Xiao-Ling;Wang Qing-Fei;Jiang Dian-Lu;Bai Juan;Tong Ru-Ting;Wang Xin-Kui   

  1. Chemistry College, Hebei Normal University, Shijiazhuang 050016;Institute of Coal Chemistry, Chinese Academy of Science, Taiyuan 030001
  • Received:2001-12-17 Revised:2002-02-10 Published:2002-08-15
  • Contact: Ding Ke-Qiang

Abstract: Calmodulin(CaM) as a major intracellular Ca2+ receptor, was self-assembled on Au electrode and investigated by electrochemical impedance spectroscopy(EIS). The results demonstrated that the self-assembly process of CaM could be divided into rapid and slow steps clearly. The influence of pH value on the new modified electrode was found to be regular, and the most packed structure appeared at the calmodulin’s isoelectric-point, the combining process of calmodulin with Ca2+ was found to be composed of three stages. In conclusion, our AC impedance study of CaM is meaningful to reveal the mechanism of CaM in the real life system.

Key words: Calmodulin, Self-assembly, pH value, Ca2+, AC impedance