Acta Phys. -Chim. Sin. ›› 2002, Vol. 18 ›› Issue (12): 1125-1128.doi: 10.3866/PKU.WHXB20021214

• Note • Previous Articles    

Thermokinetics of Activation of Metal Ions to Amylase Catalyzed Reaction

Zhang Hong-Lin;Yu Xiu-Fang;Zhang Gang   

  1. Department of Chemistry, Qufu Normal University, Qufu 273165; 1State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100
  • Received:2002-04-08 Revised:2002-07-03 Published:2002-12-15
  • Contact: Zhang Hong-Lin E-mail:Zhanghl@qfnu.edu.cn

Abstract: Power-time curves of amylase catalyzed reaction with or without activation were obtained with a 2277 thermal activity monitor(Sweden). The data of Km and vmax were calculated for enzyme-catalyzed reactions without activation. Data of apparent Km1 and vmax were calculated for activation (Ca2+, Mg2+) using thermokinetics theory and reduced extent method. From the relationship between and concentration of metal ions(c), the effects of the activation were gained for metal ions of amylase-catalyzed reaction.

Key words: Microcalorimetry, Thermokinetics, Reduced extent, Amylase, Catalyzed reaction