Acta Phys. -Chim. Sin. ›› 2003, Vol. 19 ›› Issue (09): 800-804.doi: 10.3866/PKU.WHXB20030904

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Promoter Function of Halobacterium halobium Chromosome DNA Fragment in Escherichia coli

Liu Yi;Huang Yu-Ping;Gao Zhen-Ting;Duan Zhen-Hong;Shen Ping;Qu Song-Sheng   

  1. Department of Chemistry, College of Chemistry and Molecular Sciences, 2College of Life Sciences, Wuhan University, Wuhan 430072
  • Received:2002-12-06 Revised:2003-04-01 Published:2003-09-15
  • Contact: Liu Yi

Abstract: A promoter-probe plasmid pKK232-8 was used as a vector, which functioned in eubacteria Escherichia(E.) coli HB101. The promoter function of the chromosome DNA fragment from Halobacterium(H.) halobium R1, RM13 DNA fragment, active as eubacteria promoters in E.coli HB101, was studied by microcalorimetry. The promoter is of about 1000 bp(base pair).It can promote the chloramphenicol acetyltransferase (CAT) gene in plasmid pKK232-8, and the chloramphenicol resistance level is about 150 mg•L-1.The result indicates that there are probably double-function promoters in H.halobium chromosome.

Key words: Halobacterium halobium R1, Escherichia coli HB101, Plasmid pKK232-8, Eubacterial promoter function, Microcalorimetry