Acta Phys. -Chim. Sin. ›› 2004, Vol. 20 ›› Issue (10): 1182-1185.doi: 10.3866/PKU.WHXB20041003

• Communication • Previous Articles     Next Articles

Phage Antibody Chip for Discriminating Proteomes from Different Cells

Hong Long;Liao Wei;Wei Fang;Zhao Xin-Sheng;Zhu Sheng-Geng   

  1. School of Life Science, State Key Laboratory of Molecular Dynamic and Stable Structures, and Department of Chemical Biology, College of Chemistry, Peking University, Beijing 100871
  • Received:2004-07-07 Revised:2004-07-30 Published:2004-10-15
  • Contact: Zhao Xin-Sheng

Abstract: A model analytical chip was prepared by coupling 4 phage antibody clones from mouse and 1 clone from human to the surface of carboxyl-terminated silicon. The lymphocytes from healthy donors were selected as normal cells, while HeLa cells were selected as tumor cell. The whole proteins were labeled by fluorescent dye Cy3 after isolation from lymphocytes and HeLa cells, and then reacted with the analytical chip. A different binding pattern was obtained. Our results showed that the protein chip made of phage antibodies was able to recognize the proteome from different cells.

Key words: Phage antibody library, Biosensors, Phage antibody chip, Proteome