Acta Phys. -Chim. Sin. ›› 2004, Vol. 20 ›› Issue (10): 1267-1270.doi: 10.3866/PKU.WHXB20041021

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Glucose Oxidase Modified Electrode Fabricated by Layer-by-layer Molecular Deposition

Chen Zhi-Chun;Xu Shan-Hao;Lin Han-Feng;Yang Shao-Ming;Lin Xian-Fu   

  1. Department of Chemistry ,Zhejiang University, Hangzhou 310027
  • Received:2004-04-20 Revised:2004-06-01 Published:2004-10-15
  • Contact: Lin Xian-Fu

Abstract: In this paper,glucose oxidase(GOD) electrodes were fabricated by alternative adsorption of oppositely charged polycations (poly(dimethydiallyammonium)hydrochloride, PDDA) and enzymes on Au modified by 3-mercapto-1-propanesulfonic acid, sodium salt (MPS). The concentration of glucose was detected by cyclic voltammetry via ferrocene monocarboxylic acid as mediator. A linear relationship existed between peak current and concentration of glucose in the range 0.55~6.63 mmol•L-1. The effect of pH on the response current of electroroxidation of glucose by GOD had been studied and the optimum pH was 7.0.In addition, the biosensor showed good reproducibility for the determination of glucose and the relative standard deviation was 2.4%.

Key words: Layer-by-layer assembly, Glucose oxidase (GOD), Biosensor