Acta Phys. -Chim. Sin. ›› 2005, Vol. 21 ›› Issue (05): 508-511.doi: 10.3866/PKU.WHXB20050510

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Improving Phage Antibody Chip by pⅧ Display System

LIAO Wei; HONG Long; WEI Fang; ZHU Sheng-Geng; ZHAO Xin-Sheng   

  1. State Key Laboratory of Molecular Dynamic and Stable Structures, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Peking University;    College of Life Sciences, Peking University, Beijing    100871
  • Received:2004-10-29 Revised:2004-12-10 Published:2005-05-15
  • Contact: ZHAO Xin-Sheng

Abstract: Phage antibody chips, prepared by coupling the recombinant phage displaying scFv with carboxyl-terminated silicon, can be applied in detecting proteins and proteomes. In previous work, we immobilized phages displaying scFv on the coat protein pⅢ on silicon surface as model chips. To improve the sensitivity and the ratio of signal to noise, in this work, scFv specific binding to thrombin was displayed by pⅧ display system, which is the major coat protein on the phage. As the results of changing from pⅢ to pⅧ display, the sensitivity and the ratio of signal to noise of chips were enhanced prominently, due to the increase of the copy number and the decrease of steric hindrance. It is concluded that scFv fused to the coat protein pⅧ is a promising candidate for the preparation of novel protein chips.

Key words: Protein chips, Phage-display antibody, pⅢ display system, pⅧ display system, scFv specific to thrombin