Acta Phys. -Chim. Sin. ›› 2005, Vol. 21 ›› Issue (10): 1091-1095.doi: 10.3866/PKU.WHXB20051006

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Studies on Stability of Pd(Ⅱ)Ternary Complexes and Interaction with DNA

GAO En-jun; DING Li-na; LIU Qi-tao; SUN Ya-guang   

  1. Chemistry Engineering Department, Shenyang Institute of Chemical Technology, Shenyang 110142; 1Chemical Institute of Science and Technology, Liaoning University, Shenyang 110036
  • Received:2005-02-02 Revised:2005-04-11 Published:2005-10-15
  • Contact: GAO En-jun E-mail:ejgao@yahoo.com.cn

Abstract: Two ternary complexes [Pd(bipy)(DL-gly)]Cl•2H2O(1) and [Pd(bipy)(L-lys)]Cl•H2O(2) were synthesized. The stability constants lgβ of two complexes are 13.81(1) and 13.71(2), respectively, and relative stability ΔlgK、lgX are higher than statistical value. The results show that there exists d-p π electronic effect in complexes.Intercalation of the complexes with DNA can be demonstrated by UV-Vis spectra and fluorescence spectra.The binding constsns of the two complexes with sperm DNA are 2.37×106(1) and 4.57×106(2), respectively. It shows that the interaction intensity is 1<2. The CD spectra also show that the tow complexes were reacted with DNA.The results of gel electrophoresis experiments reveal that plasmid pBR322 DNA was cleaved by the two complexes when complex concentration ranges from 3.00×10-3 to 7.50×10-4 mol •L-1, whereas insertion is main binding model when complex concentration is lower than 3.75×10-4 mol •L-1.

Key words: Palladium(Ⅱ) ternary complexes, Stability, Bipyridine, Amino acid, DNA, Insertion action