Acta Phys. -Chim. Sin. ›› 2005, Vol. 21 ›› Issue (10): 1178-1181.doi: 10.3866/PKU.WHXB20051024

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Annealing Properties of Lyoprotectant Solution

ZUO Jian-guo; HUA Ze-zhao; LIU Baolin; ZHOU Guoyan; XU Yi   

  1. Institute of Cryomedicine and Food Freezing, Shanghai University of Science and Technology, Shanghai 200093
  • Received:2005-03-18 Revised:2005-04-25 Published:2005-10-15
  • Contact: HUA Ze-zhao E-mail:tchua@sh163.net

Abstract: In order to examine the annealing behaviors of aqueous solutions of 10% tertiary butyl alcohol (TBA) and 10% sucrose in freeze- drying, a differential scanning calorimeter (DSC) has been employed to study freezing properties, annealing temperature, and annealing time. The experimental results show that when the solution is cooled, the existence of sucrose hinders the crystallization of TBA and the value of Tg′ decreases from -32.5 ℃ to -42.0 ℃, and TBA devitrifies at -30 ℃ when the solution is heated. When annealed at different temperatures around the devitrification peak, TBA all can crystallize completely and the value of Tg′ increases from -42.0 ℃ to -34.9 ℃. Annealing time depends on annealing temperature. The closer the annealing temperature is to Tg′, the longer the annealing time is. The devitrification will disappear after annealing at -37 ℃ for 20 min.

Key words: Freeze-drying, Tertiary butyl alcohol, Sucrose, Annealing, Differential scanning calorimetry, Glass transition, Devitrification