Acta Phys. -Chim. Sin. ›› 2007, Vol. 23 ›› Issue (09): 1483-1486.doi: 10.3866/PKU.WHXB20070934

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Enzymatic Activity of Candida Rugosa Lipase in an AOT/Triton X-100 Mixed Reverse Micellar System

ZHANG Yu-Xia; ZHAO Ji-Hua; DU Zhong-Yu; FANG Jian; AN Xue-Qin; SHEN Wei-Guo   

  1. College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China; College of Chemistry and Environment Science, Nanjing Normal University, Nanjing 210097, P. R. China; Department of Chemistry, East China University of Science and Technology, Shanghai 200237, P. R. China
  • Received:2007-03-22 Revised:2007-04-25 Published:2007-09-06
  • Contact: AN Xue-Qin E-mail:anxueqin@163.com

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Abstract: The catalytic activity of candida rugosa lipase has been investigated in sodium bis(2-ethylhexyl) sulfosuccinate (AOT)/Triton X-100 mixed reverse micellar systemby using castor oil as substrate. The effects of molar fraction of Triton X-100 in total surfactants, molar ratio of water to total surfactants, reaction temperature, pH and the concentration of castor oil on enzyme activity were studied when the concentration of overall surfactant was kept constant. Significant increase of the activity of candida rugosa lipase was observed in the presence of Triton X-100. It was also found that the castor oil inhibited the reaction when its concentration was higher than 0.24 mol·L-1.

Key words: Mixed reverse micelles, Candida rugosa lipase, Castor oil, AOT, Triton X-100

MSC2000: 

  • O648