Acta Phys. -Chim. Sin. ›› 2007, Vol. 23 ›› Issue (10): 1583-1588.doi: 10.3866/PKU.WHXB20071017

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The Adsorption of Bovine Serum Albumin onto Magnetic Chitosan Microspheres

ZHU Chen-Hua; SHEN He-Bai; XU Rui-Yun; WANG Hao-Yue; HAN Ji-Mei   

  1. Life and Environment Science College, Shanghai Normal University, Shanghai 200234, P. R. China; Department of Chemical Engineering, Shanghai Instiute of Technology, Shanghai 200235, P. R. China
  • Received:2007-05-21 Revised:2007-06-28 Published:2007-10-01
  • Contact: SHEN He-Bai

Abstract: Magnetic chitosan nanoparticles(Fe2O3-CS) were prepared in microemulsion by crosslinking method. The transmission electron microscopy (TEM), Fourier transforminfrared spectrophotometry (FT-IR) and vibrating specimen magnetometer (VSM) were used to characterize the nanoparticles. The mean diameter of the particles was about 40 nm with good dispersibility and magnetic response. The bovine serum albumin (BSA) was connected onto the surface of the magnetic nanoparticles with carbodiimide hydrochloride (EDC) as the activator. The products were then characterized by atomic force microscope (AFM) and ultraviolet spectrometer (UV). The results showed that the nanoparticles had a good adsorbability of the BSA. The adsorption isotherm of the BSA on the magnetic chitosan microspheres basicly obeyed the Langmuir model, with a maximum adsorption capacity of 250 mg·g -1 and an adsorption equilibrium constant of 0.007 L·mg-1. The stability of the particles-BSA complex in phosphate buffer solution (PBS) with different pH was also studied. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that BSA was desorbed frommagnetic particles under alkaline condition.

Key words: Chitosan, Magnetic, Microemulsion, Bovine serumalbumin, Adsorption


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