Acta Phys. -Chim. Sin. ›› 2008, Vol. 24 ›› Issue (04): 715-719.doi: 10.3866/PKU.WHXB20080430

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Interaction between Malachite Green and Bovine Serum Albumin

CAO Tuan-Wu; YANG Ji-Dong   

  1. Department of Chemistry and Environment, Yangtze Normal University, Chongqing 408100, P. R. China
  • Received:2007-08-28 Revised:2007-12-26 Published:2008-04-07
  • Contact: CAO Tuan-Wu E-mail:kyhua2001@yahoo.com.cn

Abstract: The interaction between malachite green (MG) and bovine serum albumin (BSA) was investigated via fluorescence and ultraviolet-visible absorption spectra in buffer solutions at different temperatures. The experimental results showed that static quenching was involved in addingMGin BSAsolution. The binding constants (KA) were 7.69×104 L·mol-1 (10 ℃), 5.31×104 L·mol-1 (20 ℃), and 4.85×104 L·mol-1 (37 ℃), respectively, and they reacted at a molar ratio of 1:1. The energy transfer efficiency E was 0.1635 and the binding distance r was 2.30 nm between MG and BSA according to Forster non-radiative energy transfer mechanism. The interaction between MG and BSA was driven mainly by hydrogen bonds and Vander Waals force according to thermodynamic parameters.

Key words: Malachite green, Bovine serumalbumin, Fluorescence quenching, Interaction

MSC2000: 

  • O644