Acta Phys. -Chim. Sin. ›› 2009, Vol. 25 ›› Issue (10): 2061-2067.doi: 10.3866/PKU.WHXB20091037

• ARTICLE • Previous Articles     Next Articles

Immobilization and Direct Electrochemistry of Horseradish Peroxidase on Large Cage-Like Mesoporous Silica FDU-12

MA Guo-Xian, ZHONG Qing-Dong, LU Xiong-Gang, LU Tian-Hong   

  1. School of Materials Science and Engineering, Shanghai University, Shanghai 200072, P. R. China|College of Chemistry and Environment Science, Nanjing Normal University, Nanjing 210097, P. R. China
  • Received:2009-05-11 Revised:2009-07-03 Published:2009-09-29
  • Contact: MA Guo-Xian E-mail:maguoxian@shu.edu.cn

Abstract:

We report on a direct electron transfer and bioelectrocatalysis of horseradish peroxidase (HRP) immobilized in mesoporous silica FDU-12 with three-dimensional (3D) large cages and entrances. UV-Vis spectroscopy, Fourier transform infrared (FTIR) spectroscopy, and impedance spectroscopy were used to prove the interaction between HRP and FDU-12. Results from cyclic voltammetry show that immobilized HRP can undergo a direct quasi-reversible electrochemical reaction. Its formal potential, E0', is -0.325 V in a phosphate buffer solution (PBS, pH 6.9) and this is almost independent of the scan rate from 40 to 300 mV·s -1. The experimental results also demonstrate that the immobilized HRP retains its bioelectrocatalytic activity for the reduction of H2O2. Furthermore, the electrode can be stored at 4 °C for several weeks without any loss of enzyme activity. Thus, FDU-12 is a novel matrix for realizing a direct electron transfer of various proteins and enzymes and the preparation of electrodes for the third biosensors.

Key words: Horseradish peroxidase, FDU-12, Direct electrochemisrty, Hydrogen peroxide, Biosensor

MSC2000: 

  • O646