微乳液成核-离子交联制备阿司匹林/壳聚糖纳米微球及其体外释放行为

doi:10.3866/PKU.WHXB20100842

Abstract

Abstract:

Chitosan (CS) nanosphere loaded aspirin (aspirin/CS) was prepared by nucleation and ionic crosslinking in an emulsion used for medical and pharmaceutical applications. Chemical component, morphology, size distribution, and crystal structure of nanospheres were characterized by Fourier transforminfrared (FTIR) spectroscopy, field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), dynamic laser light scattering (DLLS), and X-ray powder diffraction (XRD). Results showed that the diameter of a typical aspirin/CS nanosphere is about 88 nm and the distribution is uniform. The crystal structure of CS does not change during the nucleation process. The crystallinity of aspirin is dramatically reduced and aspirin is almost amorphous in the nanosphere. The drug content (mass fraction), the drug loading efficiency, and the in vitro release profiles under different conditions were investigated using UV-Vis spectrophotometry. Results showed that the drug content was about 55%, the drug loading efficiency reached 42%, and the chitosan nanosphere displayed an excellent drug-controlled release behavior under the experimental conditions.

Key words: Chitosan, Nanosphere, Nucleation in emulsion, Drug-controlled release, Conformation

MSC2000:

O645

JIN Shu-Ping, FENG Lei, HE Wen-Yan, HAN Yu-Qi, WEI Yu-Juan. Preparation and Release Behavior in vitro of Aspirin/Chitosan Nanospheres by Nucleation and Ionic Crosslinking in Emulsion[J].Acta Phys. -Chim. Sin., 2010, 26(09): 2581-2588.

References

1. Yang, Z.; Zheng, S. Y.; Harrison, W. J.; Harder, J.; Wen, X. X.; Gelovani, J. G.; Qiao, A.; Li, C. Biomacromolecules, 2007, 8: 3422
2. Aliabadi, H. M.; Brocks, D. R.; Lavasanifar, A. Biomaterials, 2005, 26: 7251
3. Greish, K.; Sawa, T.; Fang, J.; Akaike, T.; Maeda, H. J. Control. Release, 2004, 97: 219
4. Lee, E. S.; Na, K.; Bae, Y. H. J. Control. Release, 2005, 103: 405
5. Attwood, D.; Booth, C.; Yeates, S. G.; Chaibundit, C.; Ricardo, N. M. P. S. Int. J. Pharm., 2007, 345: 35
6. Yang, Z. L.; Yang, K. W.; Li, X. R.; Liu, Y. Chin. Pharm. J., 2007, 42: 519 [杨卓理, 杨可伟, 李馨儒,刘艳.中国药学杂志, 2007, 42: 519]
7. Rijcken, C. J.; Snel, C. J.; Schiffelers, R. M.; van Nostrum, C. F.; Hennink, W. E. Biomaterials, 2007, 28: 5581
8. Calvo, P.; Remuán-López, C.; Vila-Jato, J. L.; Alonso, M. J. J. Appl. Polym. Sci., 1997, 63: 125
9. Bravo-Osuna, I.; Ponchel, G.; Vauthier, C. Eur. J. Pharm. Sci., 2007, 30: 143
10. Peppas, N. A.; Hilt, J. Z.; Khademhosseini, A.; Langer, R. Adv. Mater., 2006, 18: 1345
11. Xu, Y.; Du, Y. Int. J. Pharm., 2001, 250: 215
12. Tanima, B.; Susmita, M.; Singh, K. Int. J. Pharm., 2002, 243: 93
13. Huang, X. L.; Zhang, L. M. J. Function. Polym., 2003, 16: 594 [黄小龙,张黎明. 功能高分子学报, 2003, 16: 594]
14. He, Q. F.; Li, G. M.; Wu, H. Z.; Lu, Z. M. Chin. J. Appl. Chem., 2004, 21: 192 [何强芳,李国明,巫海珍,卢志敏. 应用化学, 2004, 21: 192]
15. Sorlier, P.; Viton, C.; Domard, A. Biomacromolecules, 2002, 3: 1336
16. Amiji, M. M. Carbohyd. Polym., 1995, 26: 211
17. Anthonsen, M. W.; V? rum, K. M.; Hermansson, A. M.; Smidsrod, O.; Brant, D. A. Carbohyd. Polym., 1994, 25: 13
18. Schatz, C.; Pichot, C.; Delair, T.; Viton, C.; Domard, A. Langmuir, 2003, 19: 9896
19. Buhler, E.; Rinaudo, M. Macromolecules, 2000, 33: 2098
20. Mladenovska, K.; Cruaud, O.; Richomme, P.; Belamie, E.; Raicki, R. S.; Venier-Julienne, M. C.; Popovski, E.; Benoit, J. P.; Goracinova, K. Int. J. Pharm., 2007, 345: 59
21. Mi, F. L.; Sug, H.W.; Shyu, S. S. Carbohydr. Polym., 2002, 48: 61
22. Li, J. F.; Zhang, L.; Li, J. F.; Zou, Q.; Yang, W. H.; Li, Y. B. Chem. J. Chin. Univ., 2008, 29: 1874 [李峻峰,张利,李钧甫, 邹琴, 杨维虎,李玉宝.高等学校化学学报, 2008, 29: 1874
23. Ren, D. W.; Wang, Y. L.; Bao, D. C.; Xie, W. Y.; Ma, X. J. Spectros. Spectr. Analy., 2006, 26: 1825 [任东文, 王一力,包德才,谢威扬,马小军. 光谱学与光谱分析, 2006, 26: 1825
24. Kim, J. H.; Lee, Y. M. Polymer, 1993, 34: 1952
25. Zeng, R.; Tu, M.; Liu, H. W.; Zhao, J. H.; Zha, Z. G.; Zhou, C. R. Carbohydr. Polym., 2009, 78: 107
26. Yamada, H.; Teradac, K.; Suryanarayanana, R. J. Pharm. Biomedic. Anal., 2010, 51: 952
27. Phadnis, N. V.; Cavatur, R. K.; Suryanarayanan, R. J. Pharm. Biomedic. Anal., 1997, 15: 929
28. Shu, X. Z.; Zhu, K. J. Int. J. Pharm., 2002, 233: 217
29. Gao, Q.; Wang, G. J.; Li,W. T. Chemistry, 2009, 72: 1 [高群, 王国建,李文涛.化学通报, 2009, 72: 1]
30. Tsaih, M. L.; Chen, R. H. Int. J. Biol. Marcromol., 1997, 20: 233
31. Shu, X. Z.; Zhu, K. J.; Song, W. H. Int. J. Pharm., 2001, 212: 19

[1]	Shiyu FENG, Hao LU, Zekun LIU, Yahui LIU, Cuihong LI, Zhishan BO. Designing a High-Performance A-D-A Fused-Ring Electron Acceptor via Noncovalently Conformational Locking and Tailoring Its End Groups [J]. Acta Physico-Chimica Sinica, 2019, 35(4): 355-360.
[2]	Wenwu XU,Yi GAO. Thiolate-Protected Hollow Gold Nanospheres [J]. Acta Phys. -Chim. Sin., 2018, 34(7): 770-775.
[3]	Bangzhi WEI,Zhiyong GUO,Fan WANG,Aimin HUANG,Lin MA. Effects of Polyethyleneimine on the Conformation and Binding Capability of Human Serum Albumin [J]. Acta Phys. -Chim. Sin., 2018, 34(2): 185-193.
[4]	Ze YU,Xiaohong LI,Yunchao LI,Mingfu YE. K⁺ Concentration-Dependent Conformational Change of Pb²⁺-Stabilized G-quadruplex [J]. Acta Phys. -Chim. Sin., 2018, 34(11): 1293-1298.
[5]	Lei HAN,Li PENG,Ling-Yun CAI,Xu-Ming ZHENG,Fu-Shan ZHANG. CH₂ Scissor and Twist Vibrations of Liquid Polyethylene Glycol——Raman Spectra and Density Functional Theory Calculations [J]. Acta Phys. -Chim. Sin., 2017, 33(5): 1043-1050.
[6]	Andrew SCHLERETH,Panae NOOMUNA,Pei GAO. Mesoscale Protein Patterning on a Self-Assembled Monolayer Coated Silicon Surface through Nanosphere Lithography [J]. Acta Phys. -Chim. Sin., 2017, 33(4): 810-815.
[7]	Hai-Long CHEN,Hong-Tao BIAN,Jun-Rong ZHENG. Determining 3D Molecular Conformations with Ultrafast Multiple-Dimensional Vibrational Spectroscopy [J]. Acta Phys. -Chim. Sin., 2017, 33(1): 40-62.
[8]	Xian-Mei MENG,Shao-Long ZHANG,Qing-Gang ZHANG. Effect of the Allosteric Inhibitor Efavirenz on HIV-1 Reverse Transcriptase by Molecular Dynamics Simulation [J]. Acta Phys. -Chim. Sin., 2016, 32(2): 436-444.
[9]	Hong-Chen SHEN,Ji-Yong DING,Li LI,Fu-Feng LIU. Effect of Y220C Mutant on the Conformational Transition of p53C Probed by Molecular Dynamics Simulation [J]. Acta Phys. -Chim. Sin., 2016, 32(10): 2620-2627.
[10]	HAYIERBIEK Kulisong,Shu-Xian ZHAO,Yang YANG,Han ZENG. Performance of Nitrogen-Doped Carbon Nanocomposite with Entrapped Enzyme-Based Fuel Cell [J]. Acta Phys. -Chim. Sin., 2015, 31(9): 1715-1726.
[11]	Dong-Feng. ZHANG,Yan. ZHANG,Hua. ZHANG,Juan-Juan. QI,Yang. SHANG,Lin. GUO. Cavity-Tunable Cu₂O Spherical Nanostructures and Their NO₂ Gas Sensing Properties [J]. Acta Phys. -Chim. Sin., 2015, 31(10): 2005-2010.
[12]	FU Zhao-Ming, WANG Ming-Yang, ZHANG Yan-Xing, ZHANG Na, YANG Zong-Xian. First-Principles Study on the Microstructure of Triple-Phase Boundaries in the Ni/Yttria-Stabilized Zirconia Anode [J]. Acta Phys. -Chim. Sin., 2014, 30(6): 1055-1060.
[13]	ZHU Li-Ying, YAN Xiao-Qin, ZHANG Hong-Man, LIN Dong-Qiang, YAO Shan-Jing, JIANG Ling. Determination of Apparent Drug Permeability Coefficients through Chitosan-Sodium Cellulose Sulfate Polyelectrolyte Complex Films [J]. Acta Phys. -Chim. Sin., 2014, 30(2): 365-370.
[14]	MA Guo-Fu, MU Jing-Jing, ZHANG Zhi-Guo, SUN Kan-Jun, PENG Hui, LEI Zi-Qiang. Preparation of Polypyrrole/Sodium Alginate Nanospheres and Their Application for High-Performance Supercapacitors [J]. Acta Phys. -Chim. Sin., 2013, 29(11): 2385-2391.
[15]	ZHANG Bing-Bing, ZHAO Cong, WANG Xue-Song, HE Lei, DU Wei-Hong. Effects of 4-Hydroxyproline Stereochemistry on α-Conotoxin Solution Conformation [J]. Acta Phys. -Chim. Sin., 2013, 29(05): 1080-1087.

Preparation and Release Behavior in vitro of Aspirin/Chitosan Nanospheres by Nucleation and Ionic Crosslinking in Emulsion

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0