Acta Phys. -Chim. Sin. ›› 2010, Vol. 26 ›› Issue (11): 2997-3001.doi: 10.3866/PKU.WHXB20101008

• COLLOID AND INTERFACE CHEMISTRY • Previous Articles     Next Articles

Interactions between N-Alkyl-N,N-di(2-hydroxyethyl)-N-methylammonium Bromide and Bovine Serum Albumin

SHAO Shuang1, HU Xiao-Huan2, YOU Jin-Zong1, LEI Qun-Fang2   

  1. 1. Department of Chemistry, Zhejiang International Studies University, Hangzhou 310012, P. R. China;
    2. Department of Chemistry, Zhejiang University, Hangzhou 310027, P. R. China
  • Received:2010-04-13 Revised:2010-07-05 Published:2010-10-29
  • Contact: SHAO Shuang, LEI Qun-Fang E-mail:sshao@zjei.net, qflei@zju.edu.cn
  • Supported by:

    The project was supported by the National Natural Science Foundation of China (20673098) and Natural Science Foundation of Zhejiang Province, China (Y4080201).

Abstract:

Interactions between a series of N-alkyl-N,N-di(2-hydroxyethyl)-N-methylammonium bromides with alkyl chain from dodecyl to cetyl and bovine serum albumin (BSA) in Tris-HCl buffer solution (pH=7.1) were studied by fluorescence spectroscopy at 298 K. Effects of the surfactant structure and BSA concentration on the interactions were examined. The interaction mechanism between BSA and surfactants at a relatively low concentration range was discussed using the Stern-Volmer equation and a model of the pseudo association constant. Each of the quaternary ammonium surfactants has a fluorescence quenching effect on BSA and leads to a blue shift of the maximum emission wavelength. The Stern-Volmer quenching constant and the pseudo association constant increase, which indicates that the interactions between surfactants and BSA become stronger, as the hydrophobic chain lengths increase.

 

Key words: Quaternary ammoniumsurfactant, Bovine serumalbumin, Fluorescence quenching, Hydrophobic interaction, Pseudo association constant

MSC2000: 

  • O647