Acta Phys. -Chim. Sin. ›› 2011, Vol. 27 ›› Issue (10): 2469-2477.doi: 10.3866/PKU.WHXB20110939

• PHYSICAL CHEMISTRY OF MATERIALS • Previous Articles     Next Articles

Enhancing the Catalytic Activity of Peroxidase by Adsorption onto Fe3O4 Magnetic Nanoparticle/Multiwalled Carbon Nanotube Composite Surfaces

GAO Yun-Yan1, LI Hai-Xia1, OU Zhi-Ze1, HAO Ping1, LI Yi2, YANG Guo-Qiang3   

  1. 1. Ministry of Education Key Laboratory of Space, Applied Physics and Chemistry, School of Science, Northwestern Polytechnical University, Xi'an 710072, P. R. China;
    2. Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China;
    3. Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China
  • Received:2011-04-10 Revised:2011-06-29 Published:2011-09-27
  • Contact: GAO Yun-Yan, OU Zhi-Ze;
  • Supported by:

    The project was supported by the National Natural Science Foundation of China (21073143), Chunhui Project from the Ministry of Education of China (Z2009-1-71002, Z2009-1-71006), and Northwestern Polytechnical University Foundation for Fundamental Research, China (JC200822, JC20100239).

Abstract: Fe3O4 magnetic nanoparticles were prepared by co-precipitation and supported on the surface of aminopyridine-grafted multiwalled carbon nanotubes (MWCNT-AP), affording a superparamagnetic Fe3O4/MWCNT-AP composite with good dispersity. The Fe3O4/MWCNT-AP composites were characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and hysteresis curve measurements. Transmission electron microscopy (TEM) experimental results suggest that the Fe3O4 nanoparticles are mainly adsorbed on the tips of the MWCNT-AP. UV-Vis absorption and fluorescence results demonstrate that horseradish peroxidase (HRP) can be physically adsorbed onto the surfaces of the Fe3O4/MWCNT-AP composite. Under acidic conditions (pH 4.0), the maximum reaction rate (vmax) value of the HRP increases 3-fold after interaction with Fe3O4/MWCNT-AP.

Key words: Carbon nanotube, Magnetic nanoparticle, Peroxidase, Nanocomposite, Superparamagnetic property


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