二棕榈酰磷脂酰胆碱/豆固醇脂质体液态有序相的结构性质:同步辐射X光衍射研究

doi:10.3866/PKU.WHXB201205171

Abstract

Abstract:

The properties of the liquid ordered (L_o) phase of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)/stigmasterol liposomes were investigated using synchrotron small-angle and wide-angle X-ray diffraction techniques. The lamellar repeat distance of the L_o phase, derived from small-angle X-ray scattering experiments, changes slightly with sterol concentration and temperature. While the d-spacings of the acyl chains, evaluated from wide-angle X-ray scattering data, show a much broader range than those of the gel and liquid-crystal phases, varying from 0.422 to 0.460 nm when the temperature increases from 30 to 52 °C. The electron density profiles show that the bilayer thickness and the water-layer thickness of the liquid-ordered phase are larger than that of the liquid-crystal phase, and the bilayer thickness of the liquid-ordered phase decreases with increasing temperature. The results of this study are of help in understanding the phase state and the ordered structure of biomembranes.

Key words: Liquid ordered phase, Lipid raft, Stigmasterol, 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine, X-ray diffraction, Small-angle scattering, Electron density

MSC2000:

O641

WU Rui-Guang, CHEN Lin, YU Zhi-Wu. Structural Properties of the Liquid Ordered Phase of Phosphatidylcholine/Stigmasterol Liposomes: A Synchrotron X-Ray Diffraction Study[J].Acta Phys. -Chim. Sin., 2012, 28(08): 2008-2014.

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Structural Properties of the Liquid Ordered Phase of Phosphatidylcholine/Stigmasterol Liposomes: A Synchrotron X-Ray Diffraction Study

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