二棕榈酰磷脂酰胆碱/豆固醇脂质体液态有序相的结构性质:同步辐射X光衍射研究

doi:10.3866/PKU.WHXB201205171

Abstract

Abstract:

The properties of the liquid ordered (L_o) phase of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)/stigmasterol liposomes were investigated using synchrotron small-angle and wide-angle X-ray diffraction techniques. The lamellar repeat distance of the L_o phase, derived from small-angle X-ray scattering experiments, changes slightly with sterol concentration and temperature. While the d-spacings of the acyl chains, evaluated from wide-angle X-ray scattering data, show a much broader range than those of the gel and liquid-crystal phases, varying from 0.422 to 0.460 nm when the temperature increases from 30 to 52 °C. The electron density profiles show that the bilayer thickness and the water-layer thickness of the liquid-ordered phase are larger than that of the liquid-crystal phase, and the bilayer thickness of the liquid-ordered phase decreases with increasing temperature. The results of this study are of help in understanding the phase state and the ordered structure of biomembranes.

Key words: Liquid ordered phase, Lipid raft, Stigmasterol, 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine, X-ray diffraction, Small-angle scattering, Electron density

MSC2000:

O641

WU Rui-Guang, CHEN Lin, YU Zhi-Wu. Structural Properties of the Liquid Ordered Phase of Phosphatidylcholine/Stigmasterol Liposomes: A Synchrotron X-Ray Diffraction Study[J].Acta Phys. -Chim. Sin., 2012, 28(08): 2008-2014.

References

(1) Sun, R. G.; Zhang, J.; Hao, C. C.; Chen, Y. Y.; Yang, Q. Chem. J. Chin. Univ. 2011, 32, 2062. [孙润广, 张静, 郝长春, 陈莹莹, 杨谦. 高等学校化学学报, 2011, 32, 2062.]
(2) Verma, S. P. Curr. Drug Targets 2009, 10, 51. doi: 10.2174/138945009787122851
(3) Orlowski, S.; Coméra, C.; Tercé, F.; Collet, X. Eur. Biophys. J.2006, 36, 869.
(4) Szabo, G.; Dolganiuc, A.; Dai, Q. J. Immunol. 2007, 178, 1243.
(5) Schroeder, R.; London, E.; Brown, D. A. Proc. Nati. Acad. Sci. U. S. A. 1994, 91, 12130. doi: 10.1073/pnas.91.25.12130
(6) Sharmin, N. A.; Brown, D. A.; London, E. Biochemistry 1997,36, 10944. doi: 10.1021/bi971167g
(7) Sinha, M.; Mishra, S.; Joshi, P. G. Eur. Biophys. J. 2003, 32,381. doi: 10.1007/s00249-003-0281-3
(8) Ipsen, J. H.; Karlstroem, G.; Mouritsen, O. G.;Wennerstroem,H.; Zuckermann, M. J. Biochim. Biophys. Acta 1987, 905, 162.doi: 10.1016/0005-2736(87)90020-4
(9) Almeida, P. F. F.; Vaz,W. L. C.; Thompson, T. E. Biochemistry1992, 31, 6739. doi: 10.1021/bi00144a013
(10) Brown, D. A.; London, E. Annu. Rev. Cell Dev. Biol. 1998, 14,111. doi: 10.1146/annurev.cellbio.14.1.111
(11) Brown, D. A.; London, E. J. Membr. Biol. 1998, 164, 103. doi: 10.1007/s002329900397
(12) Simons, K.; Toomre, D. Nat. Rev. Mol. Cell Biol. 2000, 1, 31.doi: 10.1038/35036052
(13) Peskan, T.;Westermann, M.; Oelmuller, R. Eur. J. Biochem.2000, 267, 6989. doi: 10.1046/j.1432-1327.2000.01776.x
(14) Mongrand, S.; Morel, J.; Laroche, J.; Claverol, S.; Carde, J. P.J. Biol. Chem. 2004, 279, 36277. doi: 10.1074/jbc.M403440200
(15) Borner, G. H. H.; Sherrier, D. J.;Weimar, T.; Michaelson, L. V.;Hawkins, N. D. Plant Physiol. 2005, 137, 104. doi: 10.1104/pp.104.053041
(16) Dufourc, E. J. J. Chem. Biol. 2008, 1, 63. doi: 10.1007/s12154-008-0010-6
(17) Martin, S.W.; Glover, B. J.; Davies, J. M. Trends Plant Sci.2005, 10, 263. doi: 10.1016/j.tplants.2005.04.004
(18) Demel, R. A.; Kruyff, B. D. Biochim. Biophys. Acta 1976, 457,109.
(19) Mckersie, B. D.; Lepock, J. R.; Kruuv, J.; Thompson, J. E.Biochim. Biophys. Acta 1978, 508, 197. doi: 10.1016/0005-2736(78)90325-5
(20) Xu, X. L.; Bittman, R.; Duportail, G.; Heissler, D.; Vilcheze, C.;London, E. J. Biol. Chem. 2001, 276, 33540. doi: 10.1074/jbc.M104776200
(21) McKersie, B. D.; Thompson, J. E. Plant Physiol. 1979, 63, 802.doi: 10.1104/pp.63.5.802
(22) Halling, K. K.; Slotte, J. P. Biochim. Biophys. Acta 2004, 1664,161. doi: 10.1016/j.bbamem.2004.05.006
(23) Su, Y. L.; Li, Q. Z.; Chen, L.; Yu, Z.W. Colloid Surf. APhysicochem. Eng. Aspects 2007, 293, 123. doi: 10.1016/j.colsurfa.2006.07.016
(24) Wu, R. G.;Wang, Y. R.;Wu, F. G.; Zhou, H.W.; Zhang, X. H.;Hou, J. L. J. Therm. Anal. Calorim. 2012, 109, 311. doi: 10.1007/s10973-012-2331-5.
(25) Ohvo-Rekilä, H.; Ramstedt, B.; Leppimäki, P.; Slotte, J. P. Prog. Lipid Res. 2002, 41, 66. doi: 10.1016/S0163-7827(01)00020-0
(26) Wu, R. G.; Chen, L.; Yu, Z.W.; Quinn, P. J. Biochim. Biophys. Acta 2006, 1758, 764. doi: 10.1016/j.bbamem.2006.04.017
(27) McIntosh, T. J.; Magid, A. D.; Simon, S. A. Biochemistry 1989,28, 7904. doi: 10.1021/bi00445a053
(28) Wu, F. G.; Jia, Q.;Wu, R. G.; Yu, Z.W. J. Phys. Chem. B 2011,115, 8559. doi: 10.1021/jp200733y
(29) Gao,W. Y.; Chen, L.;Wu, R. G.; Yu, Z.W.; Quinn, P. J. J. Phys. Chem. B 2008, 112, 8375. doi: 10.1021/jp712032v
(30) McIntosh, T. J.; Simon, S. A. Biochemistry 1986, 25, 4058. doi: 10.1021/bi00362a011
(31) Quinn, P. J.; Takahashi, H.; Hatta, I. Biophys. J. 1995, 68, 1374.doi: 10.1016/S0006-3495(95)80310-3
(32) Yu, Z.W.; Quinn, P. J. Biophys. J. 1995, 69, 1456. doi: 10.1016/S0006-3495(95)80015-9
(33) Gao,W. Y.; Chen, L.;Wu, F. G.; Yu, Z.W. Acta Phys. -Chim. Sin. 2008, 24, 1149. [高文颖, 陈琳, 吴富根, 尉志武. 物理化学学报, 2008, 24, 1149.] doi: 10.1016/S1872-1508(08)60050-9
(34) Clarke, J. A.; Heron, A. J.; Seddon, J. M.; Law, R. V. Biophys. J.2006, 90, 2383. doi: 10.1529/biophysj.104.056499
(35) Qin, S. S.; Yu, Z.W. Acta Phys. -Chim. Sin. 2011, 27, 213.[秦姗姗, 尉志武. 物理化学学报, 2011, 27, 213.] doi: 10.3866/PKU.WHXB20110109
(36) Tristram-Nagle, S.; Moore, T.; Petrache, H. I.; Nagle, J. F.Biochim. Biophys. Acta 1998, 1369, 19. doi: 10.1016/S0005-2736(97)00197-1
(37) Gao,W. Y.; Yu, Z.W. Chin. J. Chem. 2008, 26, 1596. [高文颖, 尉志武. 中国化学, 2008, 26, 1596.] doi: 10.1002/cjoc.200890288
(38) Hui, S.W.; He, N. B. Biochmeistry 1983, 22, 1159. doi: 10.1021/bi00274a026
(39) Matuoka, S.; Kato, S.; Hatta, I. Biophys. J. 1994, 67, 728. doi: 10.1016/S0006-3495(94)80533-8
(40) Sankaram, M. B.; Thompson, T. E. Biochemistry 1990, 29,10676. doi: 10.1021/bi00499a015
(41) Rinia, H. A.; Snel, M. M. E.; Jan, P. J. M. FEBS Lett. 2001, 501,92. doi: 10.1016/S0014-5793(01)02636-9
(42) Bach, D.; Miller, I. R. Chem. Phys. Lipids 2005, 136, 67. doi: 10.1016/j.chemphyslip.2005.04.001

[1]	Jian Li,Cong Lin,Jianhua Lin,Junliang Sun. Application of Combining X-ray Diffraction and Electron Crystallography for Determination of Complex Inorganic Crystal Structure [J]. Acta Physico-Chimica Sinica, 2020, 36(1): 1907052-0.
[2]	Julia CONTRERAS-GARCíA,Weitao YANG. Perspective: Chemical Information Encoded in Electron Density [J]. Acta Phys. -Chim. Sin., 2018, 34(6): 567-580.
[3]	Tian LU,Qinxue CHEN. Revealing Molecular Electronic Structure via Analysis of Valence Electron Density [J]. Acta Phys. -Chim. Sin., 2018, 34(5): 503-513.
[4]	Farnaz HEIDAR-ZADEH,Paul W. AYERS. Generalized Hirshfeld Partitioning with Oriented and Promoted Proatoms [J]. Acta Phys. -Chim. Sin., 2018, 34(5): 514-518.
[5]	Jun-Sheng YUAN,Zi-Yu LIU,Fei LI,Shen-Yu LI. Study of the Hydrated Structure of KCl and NaCl Mixed Solutions Using X-ray Diffraction and Raman Spectroscopy [J]. Acta Phys. -Chim. Sin., 2016, 32(5): 1143-1150.
[6]	Yue-Hong WANG,Xiao-Yan LI,Yan-Li ZENG,Ling-Peng MENG,Xue-Ying ZHANG. Topological Analyses of Electron Density on π-hole Pnicogen Bonds in PO₂X…PX₃/PH₂X (X = F, Cl, Br, CH₃, NH₂) Complexes [J]. Acta Phys. -Chim. Sin., 2016, 32(3): 671-682.
[7]	LIU Meng, LI Guo-Bao, WANG Jia-Guo, LIAO Fu-Hui, LIN Jian-Hua. Low-Temperature Synthesis of Superconductor Ba_1-xK_xBiO₃ [J]. Acta Phys. -Chim. Sin., 2014, 30(9): 1611-1615.
[8]	YANG Cheng-Xu, JU Jing, ZHAO Shi-Di, XU Hang-Yu, LIU Meng, LIAO Fu-Hui, LI Guo-Bao, LIN Jian-Hua. Synthesis of β-FeSe by Salt-Flux Method under Air Atmosphere [J]. Acta Phys. -Chim. Sin., 2013, 29(12): 2661-2666.
[9]	ZHAO Lei, WAN Shi-Gang, CHEN Cheng-Dong, LIN Yi-Ji, FANG Xue-Ming, ZHANG Hui. Mirror Symmetry Breaking and Absolute Configuration Correlations of Fe(III) Complexes with Achiral Substituted o-Iminobenzosemiquinonato Ligands [J]. Acta Phys. -Chim. Sin., 2013, 29(06): 1183-1191.
[10]	WANG Qi, WU Ya-Jing, WANG Jun, LIN Xiao. Organotemplate-Free Hydrothermal Synthesis of Isomorphously Co-Substituted Mordenite Molecular Sieve [J]. Acta Phys. -Chim. Sin., 2012, 28(09): 2108-2114.
[11]	SENTHILKUMAR Annamalai, THARINI Kumaravel, SETHURAMAN Mathur Gopalakrishnan. Steric Effect of Alkyl Substituted Piperidin-4-one Oximes for Corrosion Control of Mild Steel in H₂SO₄ Medium [J]. Acta Phys. -Chim. Sin., 2012, 28(02): 399-406.
[12]	YU Hong-Bo, JIAWen-Zhi, PU Zhi-Ying, WANG Yue-Juan, LU Ji-Qing, TENG Bo-Tao, LUO Meng-Fei. Preparation and Application of a Cr₂O₃-α-AlF₃ Catalyst with a High Specific Surface Area [J]. Acta Phys. -Chim. Sin., 2011, 27(11): 2677-2681.
[13]	LI Qing-Hai, GU Tong-Xin, YU Sheng-Song, YAO Yan, LI Bing, LI Wu. Study on the Precipitation Pathway of Nanyishan Oilfield Brine at Subzero Temperatures [J]. Acta Phys. -Chim. Sin., 2011, 27(08): 1803-1808.
[14]	LI Xiao-Yan, LIU Qun, ZHENG Shi-Jun, MENG Ling-Peng. Mechanisms and Kinetics of the HOSO+NO Reaction [J]. Acta Phys. -Chim. Sin., 2011, 27(03): 564-570.
[15]	ZHOU Yong-Quan, FANG Chun-Hui, FANG Yan. Structure of Supersaturated Aqueous SodiumPentaborate Solution [J]. Acta Phys. -Chim. Sin., 2010, 26(09): 2323-2330.

Structural Properties of the Liquid Ordered Phase of Phosphatidylcholine/Stigmasterol Liposomes: A Synchrotron X-Ray Diffraction Study

PDF

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0