非支撑磷脂双层膜制备及温度对其力学性质的影响

doi:10.3866/PKU.WHXB201304233

Abstract

Abstract:

Nanoporous silicon nitride membranes are fabricated by combining polystyrene colloidal lithography with Micro-Electro-Mechanical Systems, and then freestanding bilayer lipid membranes are constructed across the nanopores by the vesicle method. The topography and mechanical properties of the freestanding bilayer lipid membranes are investigated using the imaging and force curve modes of variable-temperature atomic force microscopy (AFM). The fluidity and self-repair of the freestanding bilayer lipid membranes are observed by AFM, which gives enough freestanding area for further investigations. Force curve measurements demonstrate that both breakthrough and adhesion forces decrease as the temperature increases; i.e., the mechanical stability of freestanding bilayer lipid membranes decreases as the temperature rises. The breakthrough force of freestanding bilayer lipid membranes is smaller than that of supported membranes. Moreover, the adhesion force of freestanding bilayer lipid membranes varies in the opposite manner to that of supported membranes.

Key words: Atomic force microscopy, Freestanding bilayer lipid membrane, Nanoporous silicon nitride membrane, Vesicle, Temperature

MSC2000:

O647

WANG Yang, YAN Jia-Wei, ZHU Zai-Wen, ZHAO Xue-Qin, ZHONG Yun-Xing, MAO Bing-Wei. Preparation of Freestanding Bilayer Lipid Membranes and the Effect of Temperature on Their Mechanical Properties[J].Acta Phys. -Chim. Sin., 2013, 29(07): 1588-1594.

References

(1) Nielsen, C. H. Anal. Bioanal. Chem. 2009, 395, 697. doi: 10.1007/s00216-009-2960-0
(2) Ma,W. B.; Zhang, L. Z. Chem. Ind. Eng. Pro. 2007, 26, 1538.[马文宝, 张立志. 化工进展, 2007, 26, 1538.]
(3) Wang, L. G.; Li, Y. H.; Tien, H. T. Bioelectrochem. Bioenerg.1995, 36, 145.
(4) Yabuki, S.; Mizutani, F.; Hirata, Y. J. Electroanal. Chem. 1999,468, 117. doi: 10.1016/S0022-0728(99)00088-1
(5) Yang, C. Y.; Liu, Y.; Li, Q. G.; Li, L.W. Acta Phys. -Chim. Sin.2007, 23, 635. [杨昌英, 刘义, 李强国, 李林蔚. 物理化学学报, 2007, 23, 635.] doi: 10.1016/S1872-1508(07)60040-0
(6) Hao, C. C.; Sun, R. G.; Zhang, J. Chem. J. Chin. Univ. 2009, 30,913. [郝长春, 孙润广, 张静. 高等学校化学学报, 2009, 30,913.]
(7) Han, X. J.; Song, Y. H.;Wu, Z. Y.; Zhang, B. L.;Wang, E. K.Chem. J. Chin. Univ. 2001, 22, 1298. [韩晓军, 宋永海, 吴正岩, 张柏林, 汪尔康. 高等学校化学学报, 2001, 22, 1298.]
(8) Mabrey, S.; Sturtevant, J. M. Proc. Natl. Acad. Sci. 1976, 73,3862. doi: 10.1073/pnas.73.11.3862
(9) Janiak, M. J.; Small, D. M.; Shipley, G. G. J. Biol. Chem. 1979,254, 6068.
(10) Cheng, Y.; Bushby, R. J.; Evans, S. D.; Knowles, P. R.; Miles,R. E.; Ogier, S. D. Langmuir 2001, 17, 1240. doi: 10.1021/la001423h
(11) Steltenkamp, S.; Müller, M. M.; Deserno, M.; Hennesthal, C.;Steinem, C.; Janshoff, A. Biophys. J. 2006, 91, 217. doi: 10.1529/biophysj.106.081398
(12) Mey, I.; Stephan, M.; Schmitt, E. K.; Müller, M. M.; Ben Amar,M.; Steinem, C.; Janshoff, A. J. Am. Chem. Soc. 2009, 131,7031. doi: 10.1021/ja809165h
(13) Dufrene, Y. F.; Boland, T.; Schneider, J.W.; Barger,W. R.; Lee,G. U. Faraday Discuss. 1998, 111, 79.
(14) Dufrene, Y. F.; Barger,W. R.; Green, J. B.; Lee, G. U. Langmuir1997, 13, 4779. doi: 10.1021/la970221r
(15) Garcia-Manyes, S.; Lorena, R. M.; Gerard, O.; Sanz, F. J. Am. Chem. Soc. 2010, 132, 12874. doi: 10.1021/ja1002185
(16) Garcia-Manyes, S.; Oncins, G.; Sanz, F. Biophys. J. 2005, 89,1812. doi: 10.1529/biophysj.105.064030
(17) Garcia-Manyes, S.; Oncins, G.; Sanz, F. Biophys. J. 2005, 89,4261. doi: 10.1529/biophysj.105.065581
(18) Seeger, H. M.; Cerbo, A. D.; Alessandrini, A.; Facci, P. J. Phys. Chem. B 2010, 114, 8926. doi: 10.1021/jp1026477
(19) Keller, D.; Larsen, N. B.; Moller, I. M.; Mouritsen, O. G.Physical Review Lettlers 2005, 94, 025701. doi: 10.1103/PhysRevLett.94.025701
(20) Leonenko, Z. V.; Finot, E.; Ma, H.; Dahms, T. E.; Cramb, D. T.Biophys. J. 2004, 86, 3783. doi: 10.1529/biophysj.103.036681
(21) Seeger, H. M.; Marino, G.; Alessandrini, A.; Facci, P. Biophys. J. 2009, 97, 1067. doi: 10.1016/j.bpj.2009.03.068
(22) Charrier, A.; Thibaudau, F. Biophys. J. 2005, 89, 1094. doi: 10.1529/biophysj.105.062463
(23) Li, J.; Stein, D.; McMullan, C.; Branton, D.; Aziz, M. J.;Golovchenko, J. A. Nature 2001, 412, 166. doi: 10.1038/35084037
(24) Romer,W.; Steinem, C. Biophys. J. 2004, 86, 955.
(25) Zhang, X.; Zhu, Z.W.; Sun, C. F.; Zhu, F.; Luo, Z. Z.; Yan, J.W.; Mao, B.W. Microchim. Acta 2009, 167, 135. doi: 10.1007/s00604-009-0216-5
(26) Yu, Z.W.; Quinn, P. J. Biophys. J. 1995, 69, 1456.

[1]	Yizhou WANG,Yehong LIU,Shouhong XU,Honglai LIU. Design and Synthesis of Multi-Responsive Copolymers for Drug Carrier [J]. Acta Physico-Chimica Sinica, 2019, 35(8): 876-884.
[2]	Kang YANG,Xiaorui SHUAI,Huachao YANG,Jianhua YAN,Kefa CEN. Electrochemical Performance of Activated Graphene Powder Supercapacitors Using a Room Temperature Ionic Liquid Electrolyte [J]. Acta Phys. -Chim. Sin., 2019, 35(7): 755-765.
[3]	Junjiang GUO,Shiyun TANG,Rui LI,Ningxin TAN. Mechanism Construction and Simulation for Combustion of Large Hydrocarbon Fuels Applied in Wide Temperature Range [J]. Acta Phys. -Chim. Sin., 2019, 35(2): 182-192.
[4]	Fan ZHANG,Zhe REN,Shenghui ZHONG,Mingfa YAO,Zhijun PENG. Role of Low-Temperature Fuel Chemistry on Turbulent Flame Propagation [J]. Acta Phys. -Chim. Sin., 2019, 35(2): 158-166.
[5]	Hui LI,Shuangyu LIU,Huiming WANG,Bo WANG,Peng SHENG,Li XU,Guangyao ZHAO,Huitao BAI,Xin CHEN,Yuliang CAO,Zhongxue CHEN. Improved Sodium Storage Performance of Na_0.44MnO₂ Cathode at a High Temperature by Al₂O₃ Coating [J]. Acta Physico-Chimica Sinica, 2019, 35(12): 1357-1364.
[6]	Paul W. AYERS,Mel LEVY. Levy Constrained Search in Fock Space: An Alternative Approach to Noninteger Electron Number [J]. Acta Phys. -Chim. Sin., 2018, 34(6): 625-630.
[7]	Marco FRANCO-PÉREZ,José L. GÁZQUEZ,W. AYERS Paul,Alberto VELA. Thermodynamic Dual Descriptor [J]. Acta Phys. -Chim. Sin., 2018, 34(6): 683-391.
[8]	Ágnes NAGY. Phase Space View of Ensembles of Excited States [J]. Acta Phys. -Chim. Sin., 2018, 34(5): 492-496.
[9]	Liang XIN,Huai SUN. On the Simulation of Complex Reactions Using Replica Exchange Molecular Dynamics (REMD) [J]. Acta Phys. -Chim. Sin., 2018, 34(10): 1179-1188.
[10]	Wen-Rong ZHAO,Jing-Cheng HAO. Vesicle Gels of Magnetic Asymmetric Surfactants [J]. Acta Phys. -Chim. Sin., 2017, 33(8): 1655-1664.
[11]	Xue-Hui HUANG,Xiao-Hui SHANG,Peng-Ju NIU. Surface Modification of SBA-15 and Its Effect on the Structure and Properties of Mesoporous La_0.8Sr_0.2CoO₃ [J]. Acta Phys. -Chim. Sin., 2017, 33(7): 1462-1473.
[12]	Andrew SCHLERETH,Panae NOOMUNA,Pei GAO. Mesoscale Protein Patterning on a Self-Assembled Monolayer Coated Silicon Surface through Nanosphere Lithography [J]. Acta Phys. -Chim. Sin., 2017, 33(4): 810-815.
[13]	Qian YAO,Li-Juan PENG,Ze-Rong LI,Xiang-Yuan LI. Accurate Calculation of the Energy Barriers and Rate Constants of Hydrogen Abstraction from Alkanes by Hydroperoxyl Radical [J]. Acta Phys. -Chim. Sin., 2017, 33(4): 763-768.
[14]	Kang YUAN,Xue ZHOU,Jian-Zhong DU. Synthesis and Characterization of Thermo-Responsive Polypeptide-Based Vesicles with Photo-Cross-Linked Membranes [J]. Acta Phys. -Chim. Sin., 2017, 33(4): 656-660.
[15]	Rui GUO,Jialin ZHANG,Songtao ZHAO,Xiaojiang YU,Shu ZHONG,Shuo SUN,Zhenyu LI,Wei CHEN. LT-STM Investigation of the Self-Assembled F₁₆CuPc-Corannulene Binary System on Ag (111) and Grap [J]. Acta Phys. -Chim. Sin., 2017, 33(3): 627-632.

Preparation of Freestanding Bilayer Lipid Membranes and the Effect of Temperature on Their Mechanical Properties

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10