Acta Phys. -Chim. Sin. ›› 2015, Vol. 31 ›› Issue (11): 2043-2048.doi: 10.3866/PKU.WHXB201509141

• THERMODYNAMICS, KINETICS, AND STRUCTURAL CHEMISTRY • Previous Articles     Next Articles

Determination of Iron Liposome/Water Partition Coefficients and Identification of Influencing Factors

Ni-Na. LI1,Li-Na. GENG1,*(),Lin. WANG1,Lin-Lin. YUAN1,Yan-Zhong. CHANG2,Jian-Jun. ZHANG2   

  1. 1 College of Chemistry and Material Science, Hebei Normal University, Shijiazhuang 050024, P. R. China
    2 Testing and Analysis Center, Hebei Normal University Shijiazhuang 050024, P. R. China
  • Received:2015-05-15 Published:2015-11-13
  • Contact: Li-Na. GENG E-mail:genglina0102@126.com
  • Supported by:
    the National Natural Science Foundation of China(31201305);Natural Science Foundation of Hebei ProvinceShijiazhuang Pharmaceutical Group (CSPC) Foundation, China(C2012205082)

Abstract:

Ferric citrate liposomes (FAC-Lip) and heme liposomes (Heme-Lip) were successfully prepared by a rotary-evaporated film-ultrasonication method. The release of iron liposomes were studied in vitro, and results showed that both iron liposomes had sustained-released properties, with Heme-Lip showing superior sustained-released over FAC-Lip. The liposome/water partition coefficients (P) were determined by equilibrium dialysis and the influences on P were evaluated, as well as the binding Gibbs free energy between FAC (Heme) and liposome. The results show that P initially increased and then decreased with increasing cholesterol content and the ratio of lipid to drug. The hydrogen and electrostatic interactions were largest when P was at its maximum. At the dialysis equilibrium, the binding Gibbs free energies (ΔG) of FAC-Lip and Heme-Lip were -12.7 and -18.2 kJ·mol-1, respectively.

Key words: Ferric citrate, Heme, Liposome/water partition coefficient, Equilibrium dialysis, Gibbs free energy

MSC2000: 

  • O642.1