Acta Phys. -Chim. Sin. ›› 2016, Vol. 32 ›› Issue (12): 2951-2960.doi: 10.3866/PKU.WHXB201609231

• ARTICLE • Previous Articles     Next Articles

The Interactions between Quaternary Ammonium Cationic Surfactants and Bovine Serum Albumin

Hu-Jun XIE1,*(),Cheng-Cheng LIU1,Qiang SUN1,Qing GU2,*(),Qun-Fang LEI3,Wen-Jun FANG3,*()   

  1. 1 Department of Applied Chemistry, Zhejiang Gongshang University, Hangzhou 310018, P. R. China
    2 School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, P. R. China
    3 Department of Chemistry, Zhejiang University, Hangzhou 310028, P. R. China
  • Received:2016-07-18 Published:2016-11-30
  • Contact: Hu-Jun XIE,Qing GU,Wen-Jun FANG E-mail:hujunxie@gmail.com;guqing2002@hotamail.com;fwjun@zju.edu.cn
  • Supported by:
    The project was supported by the National Natural Science Foundation of China(21203166,21473157);Natural Science Foundation of ZhejiangProvince, China(LY16B030001);and Food Science and Engineering the Most Important Discipline of Zhejiang Province, China(JYTsp2014111)

Abstract:

UV-visible (UV-Vis) absorption spectroscopy, fluorescence spectroscopy (FL), dynamic light scattering (DLS) and isothermal titration calorimetry (ITC) were used to study the interactions between bovine serum albumin (BSA) and the three quaternary ammonium surfactants N-dodecyl-N-(2-hydroxyethyl)-N, Ndimethyl ammonium bromide (DHDAB), N-tetradecyl-N-(2-hydroxyethyl)-N, N-dimethyl ammonium bromide (THDAB) and N-cetyl-N-(2-hydroxyethyl)-N, N-dimethyl ammonium bromide (CHDAB). These surfactants quenched the intrinsic fluorescence of BSA, with longer alkyl chains resulting in more significant quenching. This was attributed to static quenching. Further evidence of static quenching was provided by UV-Vis absorption spectroscopy. The particle size of BSA was found to initially increase and then decrease with increasing surfactant concentration. The concentration of surfactant changed the type of interaction mode. This work revealed the mechanism and binding characteristics between surfactants and protein, and provides the basis for further applications of surfactants.

Key words: Surfactants, Bovine serum albumin, Fluorescence quenching, Dynamic light scattering, Isothermal titration calorimetry

MSC2000: 

  • O648