DNA的滚环扩增合成研究

doi:10.3866/PKU.WHXB201750105

Acta Phys. -Chim. Sin. ›› 2017, Vol. 33 ›› Issue (10): 2052-2057.doi: 10.3866/PKU.WHXB201750105

• ARTICLE • Previous Articles Next Articles

Study on the Synthesis of DNA via Rolling Circle Amplification

Shu-Zhen LIU,Zhi-Qing ZHANG*(),Fang WANG,Ting ZHOU,Xiu-Feng WANG,Gou-Dong ZHANG,Ting-Ting LIU,Hong-Zhi ZHANG

School of Science, China University of Petroleum(East China), Qingdao 266580, Shandong Province, P. R. China

Received:2017-03-29 Published:2017-07-17
Contact: Zhi-Qing ZHANG E-mail:zhangzq@upc.edu.cn
Supported by:
the Scientific Research Foundation for Returned Overseas Chinese Scholars(2014010615);National Natural Science Foundation of China(21603276);National Natural Science Foundation of China(21303267);Natural Science Foundation of Shandong Province, China(ZR2016BL14);Fundamental Research Funds for the Central Universities, China

Abstract

Abstract:

Rolling circle amplification (RCA) is a simple and efficient isothermal enzymatic reaction, which has developed as a novel technology in the field of nucleic acid amplification. Its product has a wide range of applications in the assembly and preparation of multi-functional materials. Here, we report the effects of reaction time and the concentrations of deoxyribonucleoside triphosphates (dNTPs), polymerase, and primer on the product of RCA. The RCA product was characterized by methods including agarose gel electrophoresis, ultraviolet spectroscopy, and transmission electron microscopy (TEM). The results showed that the length of the RCA product was significantly affected by the reaction time, especially when the reaction time was less than 30 min. With an increase of dNTPs concentrations, the concentration and chain length of the RCA product increased. However, while the concentrations of enzyme and primer had little effect on the length of the RCA product, they had a large effect on its concentration. It is worth noting that the content of the RCA product decreased significantly in the presence of excess enzyme concentration.

Key words: Rolling circle amplification, Nucleic acid, dNTPs, Polymerase, Primer

MSC2000:

O648

Shu-Zhen LIU,Zhi-Qing ZHANG,Fang WANG,Ting ZHOU,Xiu-Feng WANG,Gou-Dong ZHANG,Ting-Ting LIU,Hong-Zhi ZHANG. Study on the Synthesis of DNA via Rolling Circle Amplification[J].Acta Phys. -Chim. Sin., 2017, 33(10): 2052-2057.

References

1	Ali M. M. ; Li F. ; Zhang Z. Q. ; Zhang K. X. ; Kang D. ; Ankrum J.A. ; Le X. C. ; Zhao W. A. Chem. Soc. Rev. 2014, 43, 3324.
2	Terpe K. Appl. Microbiol. Biotechnol. 2013, 97 (24), 10243.
3	Konry T. ; Smolina I. ; Yarmush J. M. ; Irimia D. ; Yarmush M. L. Small 2011, 7 (3), 395.
4	Deng Z. X. ; Tian Y. ; Lee S. H. ; Ribbe A. E. ; Mao C. D. Angew. Chem. Int. Ed. 2005, 44, 3582.
5	Wilner O. I. ; Shimron S. ; Weizmann Y. ; Wang Z. G. ; Willner I. Nano Lett. 2009, 9 (5), 2040.
6	Hamblin G. D. ; Carneiro K. M. M. ; Fakhoury J. F. ; Bujold K. E. ; Sleiman H. F. J. Am. Chem. Soc. 2012, 134 (6), 2888.
7	Ouyang X. Y. ; Li J. ; Liu H. J. ; Zhao B. ; Yan J. ; Ma Y. Z. ; Xiao S. J. ; Song S. P. ; Huang Q. ; Jie Chao J. ; Fan C. H. Small 2013, 9 (18), 3082.
8	Yan J. ; Hu C. Y. ; Wang P. ; Liu R. ; Zuo X. L. ; Liu X. W. ; Song S. P. ; Fan C. H. ; He D. N. ; Sun G. ACS Appl. Mater. Interfaces 2014, 6 (22), 20372.
9	Linck L. ; Rei? E. ; Bier F. ; Resch-Genger U. Anal. Methods 2012, 4, 1215.
10	Xue Q. W. ; Wang Z. G. ; Wang L. ; Jiang W. Bioconjug. Chem. 2012, 23 (4), 734.
11	Akter F. ; Mie M. ; Grimm S. ; Nygren P?. ; Kobatake E. Anal. Chem. 2012, 84 (11), 5040.
12	Zhu Y. ; Wang H. J. ; Wang L. ; Zhu J. ; Jiang W. ACS Appl. Mater. Interfaces 2016, 8 (4), 2573.
13	Zhao W. A. ; Cui C. H. ; Bose S. ; Guo D. ; Shen C. ; Wong W. P. ; Halvorsen K. ; Farokhzad O. C. ; Teo G. S. L. ; Philips J. ; Dorfman D. M. ; Karnik R. ; Karp J. M. Proc. Natl. Acad. Sci. (USA) 2012, 109, 19626.
14	Chen G. ; Liu D. ; He C. B. ; Gannett T. R. ; Lin W. B. ; Weizmann Y. J. Am. Chem. Soc. 2015, 137 (11), 3844.
15	Zhang Z. Q. ; Ali M. M. ; Eckert M. A. ; Kang D. K. ; Chen Y. Y. ; Sender L. S. ; Fruman D. A. ; Zhao W. A. Biomaterials 2013, 34, 9728.
16	Zhao W. A. ; Gao Y. ; Kandadai S. A. ; Brook M. A. ; Li Y. F. Angew. Chem. Int. Ed. 2006, 45, 2409.
17	Wu Z. S. ; Zhou H. ; Zhang S. B. ; Shen G. L. ; Yu R. Q. Anal. Chem. 2010, 82 (6), 2282.
18	Kim J. H. ; Jang M. ; Kim Y. J. ; Ahn H. J. J. Med. Chem. 2015, 58 (19), 7863.
19	Ye T. ; Chen J. Y. ; Liu Y. F. ; Ji X. H. ; Zhou G. H. ; He Z. K. ACS Appl. Mater. Interfaces 2014, 6 (18), 16091.
20	Chen A. Y. ; Ma S. Y. ; Zhou Y. ; Chai Y. Q. ; Yuan R. Anal. Chem. 2016, 88 (6), 3203.
21	Ruff L. E. ; Marciniak J. Y. ; Sanchez A. B. ; Esener S. C. ; Messmer B. T. Nanotechnol. Rev. 2014, 3, 569.
22	Liu D. Y. ; Daubendiek S. L. ; Zillman M. A. ; Ryan K. ; Kool E. T. J. Am. Chem. Soc. 1996, 118 (7), 1587.
23	Zhu G. Z. ; Hu R. ; Zhao Z. L. ; Chen Z. ; Zhang X. B. ; Tan W. H. J. Am. Chem. Soc. 2013, 135 (44), 16438.
24	Lieberman K. R. ; Cherf G. M. ; Doody M. J. ; Olasagasti F. ; Kolodji Y. ; Akeson M. J. Am. Chem. Soc. 2010, 132 (50), 17961.
25	Lu J. ; Chen Y. H. ; Liu D. M. ; Ren W. ; Lu Y. Q. ; Shi Y. ; Piper J. ; Paulsen I. ; Jin D. Y. Anal. Chem. 2015, 87, 10406.
26	Chen X. Q. ; Ye Y. ; Cheng H. ; Jiang Y. W. ; Wu Y. J. Agric. Food Chem. 2009, 57, 5795.
27	Bajaj A. ; Kondaiah P. ; Bhattacharya S. Biomacromolecules 2008, 9, 991.
28	Mohsen M. G. ; Kool E. T. Acc. Chem. Res. 2016, 49 (11), 2540.
29	Lin C. X. ; Wang X. ; Liu Y. ; Seeman N. C. ; Yan H. J. Am. Chem. Soc. 2007, 129, 14475.

[1]	Yi-Fan RUAN,Nan ZHANG,Yuan-Cheng ZHU,Wei-Wei ZHAO,Jing-Juan XU,Hong-Yuan CHEN. New Developments in Photoelectrochemical Bioanalysis [J]. Acta Phys. -Chim. Sin., 2017, 33(3): 476-485.
[2]	CONG Yong, XUE Ying. Quantitative Structure-Activity Relationship Study of the Non-Nucleoside Inhibitors of HCV NS5B Polymerase by Machine Learning Methods [J]. Acta Phys. -Chim. Sin., 2013, 29(08): 1639-1647.
[3]	WANG Ya-Ping, ZHAO Xu-Hui, LU Xiang-Yu, ZUO Yu. Corrosion Protection of Ceria Particles in Mg-Rich Primer on AZ91D Magnesium Alloy [J]. Acta Phys. -Chim. Sin., 2012, 28(02): 407-413.
[4]	KANG Xue-Li, CHEN Qi-Bin, SHANG Ya-Zhuo, LIU Hong-Lai. Interaction between Gemini and DNA at the Air/Water Interface [J]. Acta Phys. -Chim. Sin., 2011, 27(06): 1467-1473.
[5]	Gao Hong-Wen;Zi Yan-Qin;Li Yu-Cheng. Langmuir Aggregation of Polychrome Blue on Nucleic Acids [J]. Acta Phys. -Chim. Sin., 2002, 18(06): 540-544.

Study on the Synthesis of DNA via Rolling Circle Amplification

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 5

Metrics

Comments

Recommended 0