Acta Phys. -Chim. Sin. ›› 1997, Vol. 13 ›› Issue (07): 650-654.doi: 10.3866/PKU.WHXB19970716

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Study on Inhibition of Xanthine Oxidase by Umbelliferone

Kan Jin-Qing,Mu Shao-Lin   

  1. Department of Chemistry,Teacher's College,Yangzhou University,Yangzhou 225002
  • Received:1996-12-16 Revised:1997-02-08 Published:1997-07-15
  • Contact: Kan Jin-Qing

Abstract:

The investigation on inhibition of xanthine oxidase by umbelliferone was carried out using a polyaniline-xanthine oxidase electrode. The experimental results indicated significant inhibition caused by umbelliferone. The apparent Michaelis-Menten constant (K'm) and the maximum rate (im) of the immobilized xanthine oxidase were both affected by umbelliferone. This indicated that umbelliferone acts as a mixed type of inhibitor of xanthine oxidase. The optimal pH of the immobilized enzyme was hardly affected by umbelliferone; The response current increases with increasing potential in the potential range 0.55~0.68V.

Key words: Polyaniline, Xanthine oxidase, Umbelliferone, Inhibition