微管蛋白聚合及其与紫杉醇作用的量热学研究

doi:10.3866/PKU.WHXB19971103

Abstract

Abstract:

The heat properties of porcine brain tubulin polymerization and the interaction of the tubulin with taxol were studied with an MS-80 Calvet Microcalorimeter .The heat vibration occured within 1.5h when tubulin solution was diluted from a highe r concentration to a lower one. The thermodynamic parameters of tubulin polymerization at 303.15K and 298. 15K were measured in 10μmol•L-1 tubulin solution, its △H's were 22kJ•mol-1 and 29.3kJ•mol-1，respectively.Adding different concentration of taxol in the course of polymerization of tubulin give rise to a great change in △H. The time of taxol combining to polymerized microtubule was about one minute after the beginning of the reaction. The mechanism of tubulin polymerization under different conditions was discussed.

Key words: Tubulin, Taxol, Thermogram

Han Gong-She,Yan Long-Fei,Wang Bao-Huai,Zhang You-Min. Microcalorimetric Studies of the Tubulin Polymerization and the Interaxtion between Tubuin and Taxol[J].Acta Phys. -Chim. Sin., 1997, 13(11): 969-973.

[1]	WU Xiao-Min, ZU Yuan-Gang, YANG Zhi-Wei, FU Yu-Jie, ZHOU Li-Jun, YANG Gang. Temperature-Controlled Molecular Dynamics Studies on the Folding Mechanism of the Tubulin Active Peptides [J]. Acta Phys. -Chim. Sin., 2009, 25(04): 773-782.
[2]	XU Zhi-Guang; XU Xuan; YUAN Chuan-Neng. Molecular Docking on Taxotere Binding to αβ-Tubulin [J]. Acta Phys. -Chim. Sin., 2008, 24(10): 1839-1844.
[3]	Zhou Xiao-Lei;Wang Bao-Huai;Li Zhi-Fen;Zeng Hui-Hui. Studies of the Effects of Anti-tumor Compounds on the Assembly and Melting Denaturation of Tubulin in vitro [J]. Acta Phys. -Chim. Sin., 2002, 18(11): 1009-1013.
[4]	Xu Xuan;Xu Zhi-Guang;Luo Yi-Fan. Ab Inito Study on δ ¹HNMR and δ ¹³CNMR of Taxol and It′s Molecular Geometry [J]. Acta Phys. -Chim. Sin., 2002, 18(05): 420-425.
[5]	Tang Hou-Kuang, He Ji, Liu Yi, Wang Cun-Xin, Xie Chang-Li, Qu song-Sheng. Growth Thermograms of Bacterial Variation Strains [J]. Acta Phys. -Chim. Sin., 1999, 15(12): 1112-1114.
[6]	Yang Yong, Wang Bao-Huai, Li Zhi-Fen, Zhang You-Min, Han Gong-She, Yan Long-Fei. The Polymerization and Denaturation of Brain Tubulin and the Effect of Taxol by Differential Scanning Calorimetry [J]. Acta Phys. -Chim. Sin., 1999, 15(02): 182-185.
[7]	Chen Xiao-Nan,Zeng Xian-Cheng,Chen Min-Zhu. Establishment of the Characteristic Thermogram Equation of Conduction Calorimeter [J]. Acta Phys. -Chim. Sin., 1996, 12(11): 1031-1035.
[8]	Zhang Guo-Xiu,Yu xiu-Fang. Study on the Thermodynamical Functions of the Hydrolytic Polymerization of Chromium(III) Ion by Microcalorimetric Method [J]. Acta Phys. -Chim. Sin., 1995, 11(08): 766-768.
[9]	Zhang Hong-Lin, Li Ji-Sheng, Nan Zhao-Dong, Sun Hai-Tao, Liu Yong-Jun, Xie Chang-Li, Qu Song-Sheng. Investigation of Optimum Growth Temperature of Bacteria by Microcalorimetric Method [J]. Acta Phys. -Chim. Sin., 1994, 10(10): 928-930.
[10]	Zeng Hui-Hui, Wang Kui, Wang Bao-Huai, Zhang You-Min. Microcalorimetry Studies on the Polymerization and the Effects of Cisplatin [J]. Acta Phys. -Chim. Sin., 1994, 10(03): 197-199.
[11]	Wang Bao-Huai, Zhang You-Min, Yang Ming, Miao Pei, Wang Kui. A Study of Intercalation Binding of Harmaline and Harmine to DNA by Microcalorimetry [J]. Acta Phys. -Chim. Sin., 1994, 10(01): 82-86.
[12]	Xu Ying-Hong; Deng Yu. Studies on Thermokinetics X. Thermogram's Peak-Heights Method for First Order Reactions [J]. Acta Phys. -Chim. Sin., 1991, 7(05): 601-604.
[13]	Xie Chang-Li; Tang Hou-Kuan; Song Zhao-Hua; Qu Song-Sheng; Liao Yao-Ting; Liu Hai-Shui. Critical Growth Temperatures of Bacteria by Microcalorimetric Method [J]. Acta Phys. -Chim. Sin., 1991, 7(04): 471-474.

Microcalorimetric Studies of the Tubulin Polymerization and the Interaxtion between Tubuin and Taxol

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 13

Metrics

Comments

Recommended 10