微管蛋白聚合及其与紫杉醇作用的量热学研究

doi:10.3866/PKU.WHXB19971103

Acta Phys. -Chim. Sin. ›› 1997, Vol. 13 ›› Issue (11): 969-973.doi: 10.3866/PKU.WHXB19971103

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Microcalorimetric Studies of the Tubulin Polymerization and the Interaxtion between Tubuin and Taxol

Han Gong-She,Yan Long-Fei,Wang Bao-Huai,Zhang You-Min

College of Biological Science,China Agricultural University,Beijing 100094|Department of Chemistry,Peking University,Beijing 100871

Received:1997-08-25 Revised:1997-09-22 Published:1997-11-15
Contact: Wang Bao-Huai

Abstract

Abstract:

The heat properties of porcine brain tubulin polymerization and the interaction of the tubulin with taxol were studied with an MS-80 Calvet Microcalorimeter .The heat vibration occured within 1.5h when tubulin solution was diluted from a highe r concentration to a lower one. The thermodynamic parameters of tubulin polymerization at 303.15K and 298. 15K were measured in 10μmol•L-1 tubulin solution, its △H's were 22kJ•mol-1 and 29.3kJ•mol-1，respectively.Adding different concentration of taxol in the course of polymerization of tubulin give rise to a great change in △H. The time of taxol combining to polymerized microtubule was about one minute after the beginning of the reaction. The mechanism of tubulin polymerization under different conditions was discussed.

Key words: Tubulin, Taxol, Thermogram

Han Gong-She,Yan Long-Fei,Wang Bao-Huai,Zhang You-Min. Microcalorimetric Studies of the Tubulin Polymerization and the Interaxtion between Tubuin and Taxol[J].Acta Phys. -Chim. Sin., 1997, 13(11): 969-973.

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Microcalorimetric Studies of the Tubulin Polymerization and the Interaxtion between Tubuin and Taxol

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