Acta Phys. -Chim. Sin. ›› 2006, Vol. 22 ›› Issue (07): 777-779.doi: 10.3866/PKU.WHXB20060702

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Recognition of Cell Surface Proteins by High-density Phage Antibody Chip

CEN Xiao-Dong;WANG Wen-Juan;ZHAO Xin-Sheng;WANG Xuan;SHEN Ti;TAN Tao-Chao;BI Qun;ZHU Sheng-Geng   

  1. College of Life Sciences, Peking University, Beijing 100871, P. R. China; State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Peking University, Beijing 100871, P. R. China; Peking Union Medical College Hospital, Beijing 100730, P. R. China
  • Received:2005-12-29 Revised:2006-02-23 Published:2006-06-27
  • Contact: ZHAO Xin-Sheng;ZHU Sheng-Geng E-mail:zhusg@pku.edu.cn;zhaoxs@pku.edu.cn

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Abstract: A large human phage antibody library was subjected for panning with leukocytes from healthy donors and leukemia patients to select for specific antibodies against leukocytic surface proteins. Recombinant phage particles displaying multiple antibody fragments via pVIII display system were used to prepare high-density phage antibody chips. Leukocyte lysate samples from 3 healthy donors and 3 leukemia patients were labeled with Cy3 fluorescence dye and reacted with the microarrays on the epoxy substrates. Eight sets of specific spots were found to have significantly distinct fluorescence intensities between the two groups of fluorescence profiles. It was demonstrated that phage antibody chips could be used for the recognition of cellular surface proteins.

Key words: Phage antibody library, pVIII display system, High-density phage antibody chip, Leukemia, Leukocytic surface protein