Acta Phys. -Chim. Sin. ›› 2009, Vol. 25 ›› Issue (03): 457-462.doi: 10.3866/PKU.WHXB20090311

• ARTICLE • Previous Articles     Next Articles

Electrochemical Reaction of Cytochrome c at Selenocystine Self-assembled Monolayers Modified Electrodes

 LIANG Min-Si, BAI Yan, LIU Min, ZHENG Wen-Jie   

  1. Department of Chemistry, Jinan University, Guangzhou 510632, P. R. China
  • Received:2008-11-13 Revised:2008-12-17 Published:2009-03-02
  • Contact: BAI Yan; ZHENG Wen-Jie E-mail:tbaiyan@jnu.edu.cn;tzhwj@jnu.edu.cn

Abstract:

Properties of selenocystine self-assembled monolayers at gold electrode (SeCys SAMs/Au) and hexadecyltrimethylammonium bromide (CTAB)-selenocystine self-assembled bilayers at a gold electrode (CTAB-SeCys SAMs/Au) were investigated by electrochemical and contact angle experiments. The electrochemical behavior of cytochrome c (Cyt c) on SeCys SAMs/Au and CTAB-SeCys SAMs/Au electrodes are discussed. We show that SeCys and CTAB-SeCys can promote the redox reaction of Cyt c on these electrodes. The promoting effect of CTAB-SeCys is stronger than of SeCys. We found that the current of the redox reaction of Cyt c increased when the concentration of CTAB increased from 1×10-5 to 1×10-4 mol·L-1. The maximumcurrent was observed when the concentration was close to the critical micelle concentration (cmc) of CTAB. The redox peaks of Cyt c on the CTAB-SeCys SAMs/Au electrode are 0.305 and 0.235 V, respectively and the electrochemical behavior is diffusion controlled. The promoting effect of SeCys was found to be due to a combination of SeCys and remnant group of lysine in Cyt c.

Key words: Selenocystine, Cytochrome c, Self-assemble, Surfactant

MSC2000: 

  • O646