Acta Phys. -Chim. Sin. ›› 2012, Vol. 28 ›› Issue (09): 2221-2230.doi: 10.3866/PKU.WHXB201206151


Preparation of Magnetic Targeted Fe3O4-TiO2Nanoparticles and Their Photocatalytic Killing Effect on Hepatoma Carcinoma Cells

WEN Wen, GAO Xiao-Ya, SONG Zhi-Ying, HAN Dong, WANG Juan, ZHU Mei-Xia, ZHANG Ai-Ping   

  1. College of Pharmacy, Shanxi Medical University, Taiyuan 030001, P. R. China
  • Received:2012-04-17 Revised:2012-06-15 Published:2012-08-02
  • Contact: ZHANG Ai-Ping
  • Supported by:

    The project was supported by the Natural Science Foundation of Shanxi Province, China (2010011048-1), Science and Technology Development Plan of Taiyuan for University Students’ Innovative and Pioneering Project in 2012, China (120164073), and Innovation Foundation of Shanxi Medical University for College Students in 2011, China.


Fe3O4-TiO2 nanoparticles with different doped amounts of Fe3O4 were prepared by three sol-gel methods at low temperature. They were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, ultraviolet-visible (UV-Vis) spectroscopy, fluorescence spectroscopy (FS), and magnetic performance. The nanoparticles which had uniform coating, good dispersion, excellent magnetism, and high photocatalytic activity were screened. The survival rates of hepatoma carcinoma cells (HepG2) were measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) cell proliferation assay, and the photo-killing effect of screened Fe3O4-TiO2 nanoparticles on HepG2 cells was investigated in different external magnetic fields. The results indicated that the core-shell structure 5% (mass fraction) Fe3O4-TiO2 nanoparticles prepared by the third method displayed good dispersion in suspension, high photocatalytic activity, and excellent magnetic responsivity. The average particle size of the 5%Fe3O4-TiO2 particles was 50 nm. Meanwhile, the photoresponsive range of TiO2 was extended to 444 nm. In the external magnetic fields, the Fe3O4-TiO2 nanoparticles excited either by ultraviolet or visible light showed no obvious difference on killing effect, while in both cases had a higher killing effect than that of nano-TiO2. Furthermore, the killing effect was enhanced with the increased magnetic field strength in the range of 0-1.0 T.

Key words: Magnetic targeting, Fe3O4-TiO2 nanoparticles, HepG2 cell, Photocatalysis, Killing effect


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