磁靶向纳米Fe3O4-TiO2复合物的制备及其对肝癌细胞的光催化杀伤效应

doi:10.3866/PKU.WHXB201206151

Abstract

Abstract:

Fe₃O₄-TiO₂ nanoparticles with different doped amounts of Fe₃O₄ were prepared by three sol-gel methods at low temperature. They were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, ultraviolet-visible (UV-Vis) spectroscopy, fluorescence spectroscopy (FS), and magnetic performance. The nanoparticles which had uniform coating, good dispersion, excellent magnetism, and high photocatalytic activity were screened. The survival rates of hepatoma carcinoma cells (HepG2) were measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) cell proliferation assay, and the photo-killing effect of screened Fe₃O₄-TiO₂ nanoparticles on HepG2 cells was investigated in different external magnetic fields. The results indicated that the core-shell structure 5% (mass fraction) Fe₃O₄-TiO₂ nanoparticles prepared by the third method displayed good dispersion in suspension, high photocatalytic activity, and excellent magnetic responsivity. The average particle size of the 5%Fe₃O₄-TiO₂ particles was 50 nm. Meanwhile, the photoresponsive range of TiO₂ was extended to 444 nm. In the external magnetic fields, the Fe₃O₄-TiO₂ nanoparticles excited either by ultraviolet or visible light showed no obvious difference on killing effect, while in both cases had a higher killing effect than that of nano-TiO₂. Furthermore, the killing effect was enhanced with the increased magnetic field strength in the range of 0-1.0 T.

Key words: Magnetic targeting, Fe₃O₄-TiO₂ nanoparticles, HepG2 cell, Photocatalysis, Killing effect

MSC2000:

O649

WEN Wen, GAO Xiao-Ya, SONG Zhi-Ying, HAN Dong, WANG Juan, ZHU Mei-Xia, ZHANG Ai-Ping. Preparation of Magnetic Targeted Fe₃O₄-TiO₂Nanoparticles and Their Photocatalytic Killing Effect on Hepatoma Carcinoma Cells[J].Acta Phys. -Chim. Sin., 2012, 28(09): 2221-2230.

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Preparation of Magnetic Targeted Fe₃O₄-TiO₂Nanoparticles and Their Photocatalytic Killing Effect on Hepatoma Carcinoma Cells

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Preparation of Magnetic Targeted Fe₃O₄-TiO₂Nanoparticles and Their Photocatalytic Killing Effect on Hepatoma Carcinoma Cells