Acta Phys. -Chim. Sin. ›› 2012, Vol. 28 ›› Issue (10): 2480-2486.doi: 10.3866/PKU.WHXB201208161

• PHYSICAL CHEMISTRY OF MATERIALS • Previous Articles     Next Articles

Nanoprobes with Enhanced Two-Photon-Sensitized Eu3+ Luminescence Properties for Live Cell Imaging

FU Xiao-Yi1,2, SHAO Guang-Sheng1, HAN Rong-Cheng1, MA Yan1, XUE Fu-Min1, YANG Fan3, FU Li-Min3, ZHANG Jian-Ping3, WANG Yuan1   

  1. 1 Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China;
    2 School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, P. R. China;
    3 Department of Chemistry, Renmin University of China, Beijing 100872, P. R. China
  • Received:2012-06-20 Revised:2012-08-16 Published:2012-09-26
  • Contact: WANG Yuan E-mail:wangy@pku.edu.cn
  • Supported by:

    The project was supported by the National Natural Science Foundation of China (21073002, 21133001, 21227803, 51121091) and National Key Basic Research Special Foundation of China (2011CB808702).

Abstract:

Luminescent nanospheres (EuPHS, dav=45 nm) containing 40% (w) of Eu(tta)3dpbt (tta= thenoyltrifluoroacetonato;dpbt=2-(N,N-diethylanilin-4-yl)-4,6-bis(3,5-dimethylpyrazol-1-yl)-1,3,5-triazine) were prepared by encapsulating Eu(tta)3dpbt in a hybrid matrix formed in situ from poly(styrene-co-methyl methacrylate), octyltrimethoxysilane, and poly(octylsiloxane). The EuPHS are promising luminescent markers for bioanalysis because of their good dispersibility in aqueous solutions, high photostability, low cytotoxicity, and bright Eu3+ luminescence under excitation at long wavelengths. EuPHS exhibited excellent visible light-sensitized and near-infrared two-photon-sensitized Eu3+ luminescence properties, with a visible-light excitation peak at 415 nm and an excitation window extending up to 475 nm. The quantum yield for Eu3+ luminescence was 0.31 (λex=415 nm, T=23 ℃), and the two-photon excitation (TPE) action cross section was 5.0×105 GM (1 GM=10-50 cm4·s·photo-1×particle-1) at 830 nm. Bionanoprobes prepared by adsorbing transferrin on the surfaces of EuPHS were successfully applied in target-specific labeling and two-photon-excitation imaging of live HeLa cells.

Key words: Luminescence, Europium complex, Two-photon excitation, Bio-nanoprobe, Cell Imaging

MSC2000: 

  • O648