Abstract: Using a home-made in situ electrochemical cell of electrochemical impedance spectroscop(EIS), the impedance behaviors of the titanium/MG63 cells interface were measured to elucidate the interfacial structure and to monitor the interactions of biomaterials and living cells in a physiological condition. The in situ electrochemical cell was designed to detect the dynamic EIS behavior of living cells growing on a tiatanium surface. A three-well design of electrochemical cell was used to decrease system error and a home-made Ag/AgCl electrode was introduced as a reference electrode to eliminate the distortion phenomena in high frequency range. The EIS spectra and its time dependency of titanium/MG63 cells interface showed that the polarization resistance was increased with the decreasing of CPE constant (Y0) and a new time constant in high frequency range was detected. It suggested that the adhesion and growth activities of the living cells on the biomaterial surface may influence the surface status and the structure of the double layer at the interface, and thus alter its electrochemical characteristics.

Key words: Titanium/cells interface, in situ EIS, Ag/AgCl reference electrode