Acta Phys. -Chim. Sin. ›› 2000, Vol. 16 ›› Issue (02): 184-187.doi: 10.3866/PKU.WHXB20000217

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Relationship between the Constitutes of DNA Double Strand Breaks and Scavenging Capacity

Shao Chun-Lin, JI  Teng-Zhen-Hong, Yu Zeng-Liang   

  1. Laboratory of lon Beam Bioengineering,Institute of Plasma Physics,the Chinese Academy of Science,Hefei 230031|Research Reaator Institute,Kyoto University,Osaka 5900494,Japan
  • Received:1999-03-24 Revised:1999-07-09 Published:2000-02-15
  • Contact: Shao Chun-Lin

Abstract:

Measured by gel electrophoresis scanning, it was shown that the double strand breaks (DSBs) of pBR322 irradiated by γ rays were constituted by two parts, αDSB and βDSB, and the relationship between α,β and scavenging capacity σ was obtained. Results showed that β/α non linearly decreased with increasing σ. Moreover, the yield of the OH free radicals reacting with DNA was analyzed.

Key words: Irradiation, pBR322, Constitutes of DSB, Mannitol