Acta Phys. -Chim. Sin. ›› 2000, Vol. 16 ›› Issue (08): 729-734.doi: 10.3866/PKU.WHXB20000811

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Interactions of Dioxycyclobutenedione-(1,2-cyclohexanediamine) Platinum(II) with Calf Thymus SNA

Zhou Li, Wang Bao-Huai, Li Zhi-Fen, Li Ce, Yang Ming   

  1. Institute of Physical Chemistry,Peking University,Beijing 100871|College of Applied Sciences and Humanities of Beijing United University,Beijing 100083|Research Laboratoriy of Natural and Biomimetic Drugs,Beijing Medical University,Beijing 100083
  • Received:1999-10-29 Revised:2000-03-22 Published:2000-08-15
  • Contact: Wang Bao-Huai

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Abstract:

The interaction of a group of new synthetic compounds, dioxycyclobutenedione- (1, 2 -cyclohexanedimine ) platinum (Ⅱ) (DCP ) and its stereoisomers, with calf thymus DNA was investigated by DSC and NMR methods. In the presence of three stereoisomers of the compounds, the melting temperatures of DNA are 350.45 (SS, trans-d-l-DCP), 349.65 (RS, cis-DCP) and 348.85(RR, trans-1-DCP) )K respectively, while the melting temperature of DNA is 351.05 K. Comparing the 13C NMR spectra of three stereoisomers of the compounds with and without DNA to that of squaric acid at pH = 7. 0 and room temperature, it can be obviously seen that square acid bases dropped out of the compound molecules as leaving group and got into the solutions as they bound to DNA. On the basis of the above experimental results, the binding strength and the model of the compounds with DNA have been discussed.

Key words: Dioxycyclobutenedione-(1,2-cyclohexanediamine) platinum(II), Calf thymus DNA, DSC, NMR spectra