Acta Phys. -Chim. Sin. ›› 2007, Vol. 23 ›› Issue (02): 237-241.doi: 10.3866/PKU.WHXB20070219

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DNA-Binding Studies of a Fluorenyl-Containing Ru(II) Complex

YAO Wei;WU Bao-Yan;GAO Li-Hua;WANG Ke-Zhi   

  1. Department of Chemistry, Beijing Normal University, Beijing 100875, P. R. China; 2School of Chemical and Environment Engineering, Beijing Technology and Business University, Beijing 100037, P. R. China
  • Received:2006-08-07 Revised:2006-09-06 Published:2007-02-01
  • Contact: WANG Ke-Zhi E-mail:kzwang@bnu.edu.cn

Abstract: A ruthenium(II) complex of [Ru(bpy)2(Hfip)](ClO4)2 (bpy=2,2′-bipyridine, Hfip=2-(9H-fluoren-2-yl)-1H-imidazolo-[4,5-f]-[1,10]-phenanthrolin) was synthesized. The DNA binding properties of the complex were studied by means of electronic absorption and emission spectroscopic titrations, steady-state emission quenching by Fe(CN)4-6, viscosity measurements, DNA competitive binding with ethidium bromide, and thermal denaturation of DNA. The results indicated that the complex bound to calf-thymus DNA in an intercalative mode with a binding constant of 8.6×105 L·mol-1 in the presence of 50 mmol·L-1 NaCl solution.

Key words: Ruthenium (II), DNA, Phenanthroline, UV-Vis spectrum, Emission spectrum