Acta Phys. -Chim. Sin. ›› 1995, Vol. 11 ›› Issue (11): 1026-1030.doi: 10.3866/PKU.WHXB19951113

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The Study on the Thermodynamic and Kinetic Behaviour for Mitochondrial Metabolism by Means of Calorimetry

Zhou Pei-Jiang, Xie Chang-Li, Liu Yi, Qu Song-Sheng, Zhu Ying-Guo, Zhou Han-Tao   

  1. Department of Chemistry,Wuhan University,Wuhan 430072|School of Life Science,Wuhan University,Wuhan 430072
  • Received:1994-12-30 Revised:1995-03-29 Published:1995-11-15
  • Contact: Xie Chang-Li

Abstract:

The metabolic thermograms and DSC curves of the mitochondria isolated from rice have been determined by using an LKB 2277 bioactivity monitor and a Dupont 910 DSC & 9900 Computer/Thermal Analyzer System. The thermodynamics and kinetics of the rice mitochondrial metabolism out of the cell have been studied. The metabolic thermograms of the rice mitochondria show different stages. If the rice mitochondria have been laid up for different times, the metabolic thermograms and kinetic behaviours of the rice mitochondria are different. Some thermodynamic values and kinetic parameters, as well as some new experimental thermokinetic equations in the activity increasing stage of the rice mitochondria, have been calculated and established. Furthermore, the thermodynamic and kinetic behaviour of the rice mitochondria in the metabolism out of the cell under changeable and low temperatures have been approached preliminarily.

Key words: Mitochondria, Metabolism, Calorimetry, Thermodyanmics, Kinetics