Acta Phys. -Chim. Sin. ›› 2002, Vol. 18 ›› Issue (08): 686-691.doi: 10.3866/PKU.WHXB20020804

• ARTICLE • Previous Articles     Next Articles

The pH Effect on the Fluorescence of Linear Poly-amino-compounds and the Recognition of Nucleoside Phosphates

Mei Ming-Hua;Yan Jing-Jing;Wu Shi-Kang   

  1. Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100101
  • Received:2001-12-06 Revised:2002-01-21 Published:2002-08-15
  • Contact: Wu Shi-Kang

Abstract: Three kinds of naphthalene containing poly-amino-compounds with different chain length have been synthesized in this work. Their fluorescence spectra were recorded under different pH conditions and showed that the fluorescence intensity of studied compounds depends on the pH value very strongly. It means that this kind of compounds can be used as a probe for the determination of pH value of solution. In the solution of these compounds, different kinds of nucleoside phosphate (ATP, ADP and AMP) were introduced respectively. It was found that the fluorescence of compounds studied in this work such as C10H7CH2(NHCH2CH2)3NH2 (compound 3) can be quenched by different nucleoside phosphate and their quenching ability which was evaluated by the Stern-Volmer constants or quenching constants were also different. The results obtained showed that the ATP has the strongest ability to the fluorescence quenching of compound 3. It demonstrated that the molecular size matching and the sterically arrangement of groups of studied compounds with nucleoside phosphates are very important for the interaction of them. And the quenching ability depends on the interaction of these species strongly. Evidently, this phenomenon can be used as a method for the molecular recognition of nucleoside phosphate by the changing of fluorescence intensity of these compounds.

Key words: Molecular recognition, Fluorescence chemical sensor, Nucleoside phosphate, Poly-amino compounds, Molecular interaction