Acta Phys. -Chim. Sin. ›› 1997, Vol. 13 ›› Issue (05): 449-451.doi: 10.3866/PKU.WHXB19970512

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The Crystallization of Acidic Phospholipase A2 from the Venom of Agkistrodon Blomhoffii Brevicaudus Covalently Inhibited by p-Bromo-phenacyl-bromide

Zhao Hai-Yan,Song Shi-Ying,Lin Zheng-Jiong,Du Xiao-Yan,Zhou Yuan-Cong   

  1. National Laboratory of Biomacromolecules,Institute of Biophysics,Academia Sinica,Beijing 100101|Shanghai Institute of Biochemistry,Academia Sinica,Shanghai 200031
  • Received:1996-08-21 Revised:1997-01-06 Published:1997-05-15
  • Contact: Zhao Hai-Yan

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Abstract:

The Crystals of acidic phospholipase A2 from the venom of Agkistrodon blomhoffii brevicaudus (i.e. Agkistrodon halys pallas) covalently modified with p-bromo-phenacylbromide were obtained by the method of hanging drop vapor diffusion. Crystallization droplet was composed of 0.06mol?L-1 Na(CH3)2AsO2 (pH=6.5), 24.8% (v/v) 1,4-butanediol, and 4.5mg?mL-1 protein. The crystal data are of a=b=82.82?., c=32.85?, space group P61. 8945 unique reflections with 1.93 ? resolution were measured on a Siemens area detector X-ray diffractometor, of which 8069 reflections having F0 >2σ(F). The experimental results show that the crystals are suitable to a structure analysis of high resolution.

Key words: Phospolipase A2, pBPB inhibition, Crystallization, X-ray diffraction