Acta Phys. -Chim. Sin. ›› 2005, Vol. 21 ›› Issue (08): 862-866.doi: 10.3866/PKU.WHXB20050808

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Interaction between Tween-20 and BSA

LIU Jing; XU Gui-ying; LIU Jun; YU Li; ZHANG Hong-lin; YU Xiu-fang   

  1. Key Laboratory of Colloid and Interface Chemistry Ministry of Education, Shandong University, Jinan 250100; Department of Chemistry, Qufu Normanl University, Qufu 273165; Pharmic Institute, Hebei Medical University, Shijiazhuang 050017
  • Received:2004-12-24 Revised:2005-02-16 Published:2005-08-15
  • Contact: XU Gui-ying E-mail:xuguiying@sdu.edu.cn

Abstract: The interaction between Tween-20 and BSA was studied by surface tension and isothermal titration microcalorimetry. The results show that there is a weak interaction between Tween-20 and BSA and the Tween-20 molecules can replace BSA molecules adsorbed on the surface. A competition adsorption between Tween-20 and BSA occurred at the surface of mixed solution. The tendency of change of the properties of the BSA/Tween-20 mixed system with temperature is similar to that of the single non-ionic surfactant. In the range of BSA concentration from 3.7×10-5 kg•L-1 to 1.1×10-5 kg•L-1, the cmc of Tween-20 does not change with BSA concentrations. When the amount of Tween-20 is fixed, the thermal effects of the interaction between BSA and Tween-20 does not depend on the BSA concentration. The thermodynamic parameters of the mixed solutions in the presence or absence of BSA indicate that the cmc of Tween-20 decreases and the value of entropy change of the system increases in the presence of BSA in solution.

Key words: Tween-20, BSA, Surface tension, Isotherm titration microcalorimetry, Interaction