Acta Phys. -Chim. Sin. ›› 2003, Vol. 19 ›› Issue (05): 385-388.doi: 10.3866/PKU.WHXB20030501

• Communication •     Next Articles

The Novel Application of Polyclonal Antibodies in Recognizing Anti-EGFR Inhibitors Directly from Herb

Zhu Li-Li;Xu Xiao-Jie   

  1. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871
  • Received:2003-02-25 Revised:2003-03-18 Published:2003-05-15
  • Contact: Xu Xiao-Jie E-mail:xiaojxu@chem.pku.edu.cn

Abstract: A polyclonal antibody (PcAb) prepared with piceatannol, a known inhibitor against the epidermal growth factor receptor (EGFR),was adopted as a stationary phase in the chromatographic system. Using the antibody to mimic the enzyme, several anti-EGFR inhibitors were recognized directly from a crude extract of Tibetan herb. Frontal affinity chromatography (FAC) was used here for analyzing molecular interactions between the analytes and an immobilized ligand (in this case the PcAb conjugated to Sepharose CL-4B) by calculating the extent of retardation of the elution front. By combining FAC with mass spectrometry (MS) in the current study, a very efficient and straightforward procedure was developed for analyzing the binding properties of different inhibitors. The novel effective screening of anti-EGFR inhibitors using PcAb from natural resources affords us a new feasible approach for the discovery of lead compounds.

Key words: Epidermal growth factor receptor (EGFR), Polyclonal antibody (PcAb),  Inhibitor, Frontal affinity chromatography (FAC)-electrospray ionization mass spectrometry(ESI-MS)