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ISSN 1000-6818CN 11-1892/O6CODEN WHXUEU
Acta Phys Chim Sin >> 2008,Vol.24>> Issue(08)>> 1359-1365     doi: 10.3866/PKU.WHXB20080806         中文摘要
Mechanism of Photokilling LoVo Cells by Nano TiO2 Photocatalytic Oxidation Combined with Immunity and Electroporation Method
SUN Yi; XU Juan; CAI Wen-Bin; JIANG Zhi-Yu
Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Department of Chemistry, Fudan University, Shanghai 200433, P. R. China; Department of Chemical Engineering, Jiangsu Polytechnic University, Changzhou 213164, Jiangsu Province, P. R. China
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The nano TiO2 photocatalytic oxidation method combined with immunity and electroporation techniques performed high efficiency for photokilling LoVo cancer cells even at very low concentration (3.12 μg·mL-1). In this method, the LoVo cells could be killed completely within 30 min under the irradiation of UV light (wavelength 253.7 nm, 4 mW·cm-2). The mechanism of this process was investigated using confocal florescent microscopy, TEM, and comet assay methods. The results demonstrated that the antibody modified nano TiO2 particles could adsorb on the cell membrane, after electroporation it could enter the inside of LoVo cells and located around the karyon. Under the irradiation of UV light, the cell’s structure, organelle, and DNA were destroyed seriously, which caused the necroses of LoVo cells. Because the photocatalysis effect of nano TiO2 took place inside of cells, so the photokilling efficiency enhanced strongly.

Keywords: Nano TiO2   LoVo cancer cell   Photocatalytic oxidation   Immunity   Electroporation   Comet assay  
Received: 2008-03-03 Accepted: 2008-04-14 Publication Date (Web): 2008-06-17
Corresponding Authors: CAI Wen-Bin; JIANG Zhi-Yu Email: zyjiang@fudan.ac.cn;wbcai@fudan.edu.cn


Cite this article: SUN Yi; XU Juan; CAI Wen-Bin; JIANG Zhi-Yu. Mechanism of Photokilling LoVo Cells by Nano TiO2 Photocatalytic Oxidation Combined with Immunity and Electroporation Method[J]. Acta Phys. -Chim. Sin., 2008,24 (08): 1359-1365.    doi: 10.3866/PKU.WHXB20080806
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