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Acta Physico-Chimica Sinica  2008, Vol. 24 Issue (08): 1359-1365    DOI: 10.3866/PKU.WHXB20080806
Article     
Mechanism of Photokilling LoVo Cells by Nano TiO2 Photocatalytic Oxidation Combined with Immunity and Electroporation Method
SUN Yi; XU Juan; CAI Wen-Bin; JIANG Zhi-Yu
Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Department of Chemistry, Fudan University, Shanghai 200433, P. R. China; Department of Chemical Engineering, Jiangsu Polytechnic University, Changzhou 213164, Jiangsu Province, P. R. China
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Abstract  The nano TiO2 photocatalytic oxidation method combined with immunity and electroporation techniques performed high efficiency for photokilling LoVo cancer cells even at very low concentration (3.12 μg·mL-1). In this method, the LoVo cells could be killed completely within 30 min under the irradiation of UV light (wavelength 253.7 nm, 4 mW·cm-2). The mechanism of this process was investigated using confocal florescent microscopy, TEM, and comet assay methods. The results demonstrated that the antibody modified nano TiO2 particles could adsorb on the cell membrane, after electroporation it could enter the inside of LoVo cells and located around the karyon. Under the irradiation of UV light, the cell’s structure, organelle, and DNA were destroyed seriously, which caused the necroses of LoVo cells. Because the photocatalysis effect of nano TiO2 took place inside of cells, so the photokilling efficiency enhanced strongly.

Key wordsNano TiO2      LoVo cancer cell      Photocatalytic oxidation      Immunity      Electroporation      Comet assay     
Received: 03 March 2008      Published: 17 June 2008
MSC2000:  O643  
  O644  
Corresponding Authors: CAI Wen-Bin; JIANG Zhi-Yu     E-mail: zyjiang@fudan.ac.cn;wbcai@fudan.edu.cn
Cite this article:

SUN Yi; XU Juan; CAI Wen-Bin; JIANG Zhi-Yu. Mechanism of Photokilling LoVo Cells by Nano TiO2 Photocatalytic Oxidation Combined with Immunity and Electroporation Method. Acta Physico-Chimica Sinica, 2008, 24(08): 1359-1365.

URL:

http://www.whxb.pku.edu.cn/Jwk_wk/wlhx/10.3866/PKU.WHXB20080806     OR     http://www.whxb.pku.edu.cn/Jwk_wk/wlhx/Y2008/V24/I08/1359

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