In this paper, the synthesis of α, ω-azobenzen, viologen bichromophore long chain compounds, and the study on their photochemical and photophysical properties have been reported. The results indicated that the photoisomerization of azobenzene could regulat the constant of complexation between the viologen and eosin, and the photoregulating effect is correlated strongly with the length of the polymethylene spacer between two chromophores. Based on the spectra data of 1H NMR, the conformation effect has also been discussed and explained preliminarily.