Acta Phys. -Chim. Sin. ›› 2010, Vol. 26 ›› Issue (01): 18-22.doi: 10.3866/PKU.WHXB20100119

• CHEMICAL KINETICS AND MOLECULE DYNAMICS • Previous Articles     Next Articles

Recombination Process between Myoglobin and NO Studied by Time-Resolved Raman Spectroscopy

LI Tao, LV Rong, YU An-Chi   

  1. Department of Chemistry, Renmin University of China, Beijing 100872, P. R. China
  • Received:2009-09-14 Revised:2009-10-27 Published:2009-12-29
  • Contact: YU An-Chi E-mail:a.yu@chem.ruc.edu.cn

Abstract:

Nanosecond transient Raman spectroscopy is a widely used technique to investigate ultrafast structural dynamics in molecules. We studied the rebinding kinetics between deoxymyoglobin (DeoxyMb) and NO using nanosecond transient Raman spectroscopy. By monitoring the ratio between the intensity of the υ4 vibrational mode in the photoproduct (DeoxyMb) and in the reactant (MbNO) at different incident laser fluxes, we obtained the recombination kinetics between DeoxyMb and NO in MbNO. For comparison, the kinetics of NO rebinding to DeoxyMb in MbNO was also studied using picosecond time-resolved Raman and absorption experiments. The kinetics results obtained with nanosecond transient Raman were consistent with those obtained by picosecond time-resolved Raman and absorption experiments.

Key words: Nanosecond transient Raman, Picosecond time-resolved Raman, Picosecond time-resolved absorption, Myoglobin, NO, Recombination

MSC2000: 

  • O643